Department of Emergency, Zhuzhou Central Hospital, Zhuzhou, China.
Department of Neurology, Zhuzhou Central Hospital, Zhuzhou, China.
Medicine (Baltimore). 2021 Oct 15;100(41):e27535. doi: 10.1097/MD.0000000000027535.
Enhancer RNAs (eRNAs), a subclass of lncRNAs, are derived from enhancer regions. The function of eRNAs has been reported by many previous studies. However, the role of eRNAs in gastric cancer, especially the prognosis-associated eRNAs, has not been studied yet.In this study, we have used a novel approach to screened key eRNAs in gastric cancer. Kaplan-Meier correlation analysis and Co-expression analysis were used to find the most significant survival-associated eRNAs. Enrichment analysis is applied to explore the key functions and pathways of screened eRNAs. The correlation and survival analysis are used to evaluate targeted genes in the pan-cancer analysisA total of 63 prognostic-associated eRNAs in gastric cancer were identified, the top 6 eRNAs were LINC01714, ZNF192P1, AC079760.2, LINC01645, EMX2OS, and AC114489.2. The correlation analysis demonstrated the top 10 screened eRNAs and their targeted genes. The results demonstrated that EMX2OS was ranked as the top eRNA according to the results of the Kaplan-Meier analysis. The correlation analysis demonstrated that eRNA EMX2OS is correlated with age, grade, stage, and cancer status. The pan-cancer analysis demonstrated that EMX2OS was associated with poor survival outcomes in adrenocortical carcinoma, cervical squamous cell carcinoma and endocervical adenocarcinoma, kidney renal clear cell carcinoma, stomach adenocarcinoma, and uveal melanoma.In this study, survival-related eRNAs were screened and the correlation between survival-related eRNAs and their targeted genes was demonstrated. EMX2OS plays a prognosis-associated eRNA role in gastric cancer, which might be a novel therapeutic target in clinical practice.
增强子 RNA（eRNAs）是 lncRNA 的一个子类，来源于增强子区域。许多先前的研究已经报道了 eRNAs 的功能。然而，eRNAs 在胃癌中的作用，特别是与预后相关的 eRNAs，尚未得到研究。在这项研究中，我们使用了一种新的方法来筛选胃癌中的关键 eRNAs。Kaplan-Meier 相关性分析和共表达分析用于寻找最显著的与生存相关的 eRNAs。富集分析用于探索筛选出的 eRNAs 的关键功能和途径。相关性和生存分析用于评估泛癌分析中靶向基因。共鉴定出 63 个与胃癌相关的预后相关 eRNAs，前 6 个 eRNAs 是 LINC01714、ZNF192P1、AC079760.2、LINC01645、EMX2OS 和 AC114489.2。相关性分析显示了前 10 个筛选出的 eRNAs 及其靶向基因。结果表明，根据 Kaplan-Meier 分析结果，EMX2OS 是排名最高的 eRNA。相关性分析表明，eRNA EMX2OS 与年龄、分级、分期和癌症状态相关。泛癌分析表明，EMX2OS 与肾上腺皮质癌、宫颈鳞状细胞癌和宫颈内膜腺癌、肾透明细胞癌、胃腺癌和葡萄膜黑色素瘤的不良生存结局相关。在这项研究中，筛选了与生存相关的 eRNAs，并证明了与生存相关的 eRNAs 及其靶向基因之间的相关性。EMX2OS 在胃癌中发挥着与预后相关的 eRNA 作用，这可能是临床实践中的一个新的治疗靶点。
