Epstein J, Kirdani R Y
J Med. 1986;17(1):51-6.
We evaluated the formation of DNA-DNA cross links by busulfan as an assay for determining the sensitivity of cells to the drug. HL-60 cells were incubated with busulfan and the effects of the drug on cell growth were compared with the effects of the drug on the alkaline elution pattern of the DNA. While incubation with 100 micrograms/ml for 1 h inhibited cell growth by 50%, drug concentrations of up to 500 micrograms/ml had no effect on the elution pattern of the DNA.
我们评估了白消安诱导的DNA-DNA交联形成情况，以此作为一种测定细胞对该药物敏感性的检测方法。将HL-60细胞与白消安一起孵育，并将该药物对细胞生长的影响与对DNA碱性洗脱模式的影响进行比较。虽然在100微克/毫升的浓度下孵育1小时会使细胞生长抑制50%，但高达500微克/毫升的药物浓度对DNA的洗脱模式没有影响。
