Choi Yun Ju, Park Jun Woo, Lim Yong, Seo Sungbaek, Hwang Dae Youn
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea.
Department of Clinical Laboratory Science, College of Nursing and Healthcare Science, Dong-Eui University, Busan, Republic of Korea.
Nanotoxicology. 2021 Nov;15(9):1180-1198. doi: 10.1080/17435390.2021.1996650. Epub 2021 Nov 3.
To assess the impact of nanoplastics (NP) and coagulation-based purified NP (PurNP), this study analyzed for alterations in the biodistribution, toxicity and inflammatory response in ICR mice exposed to three different doses of NP (5, 25, and 50 mg/kg) and PurNP for 2 weeks. Except water consumption, which was dose-dependently and significantly increased in all NP-treated groups, most factors assessed for feeding behaviors and excretions remained constant, without any significant change. Orally administered NP was detected in the intestine, kidneys, and liver at all concentrations, although the accumulation was higher in the intestine than in the kidneys and liver. No significant alterations were detected in the levels of serum biochemical markers and histopathological structures. However, compared to the vehicle group, expressions of the inflammatory response proteins (iNOS and COX-2) and mRNA levels of the inflammatory cytokines were remarkably increased in the liver, kidneys, and intestine of NP-treated mice. A similar increase was detected in the oxidative stress responses, including ROS concentration, SOD activity, and Nrf2 expression. Furthermore, similar inflammatory responses were observed in the PurNP-treated group, as compared to the vehicle-treated group. The results presented in this study provide the first strong evidence that oral administration of NP for 2 weeks results in high accumulation in the liver, kidneys, and intestine of ICR mice, and induces severe inflammatory and oxidative stress responses. These results additionally confirm the efficacy of water purification using the tannic acid-mediated coagulation removal technique.
为评估纳米塑料（NP）和基于凝聚法的纯化纳米塑料（PurNP）的影响，本研究分析了暴露于三种不同剂量（5、25和50mg/kg）的NP和PurNP两周的ICR小鼠的生物分布、毒性和炎症反应的变化。除了所有NP处理组的饮水量呈剂量依赖性显著增加外，评估的大多数摄食行为和排泄物相关因素保持不变，无任何显著变化。在所有浓度下，肠道、肾脏和肝脏中均检测到口服给药的NP，尽管肠道中的积累量高于肾脏和肝脏。血清生化标志物水平和组织病理学结构未检测到显著变化。然而，与溶剂对照组相比，NP处理小鼠的肝脏、肾脏和肠道中炎症反应蛋白（iNOS和COX-2）的表达以及炎症细胞因子的mRNA水平显著增加。在氧化应激反应中也检测到类似的增加，包括ROS浓度、SOD活性和Nrf2表达。此外，与溶剂处理组相比，PurNP处理组也观察到类似的炎症反应。本研究结果提供了首个有力证据，即ICR小鼠口服NP两周会导致其肝脏、肾脏和肠道中大量积累，并引发严重的炎症和氧化应激反应。这些结果还证实了使用单宁酸介导的凝聚去除技术进行水净化的有效性。
