靶向诱导点亮 RNA 适体转录构建新型碱性磷酸酶检测方法。
Target-induced transcription of a light-up RNA aptamer to construct a novel method for alkaline phosphatase assay.
机构信息
Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
出版信息
Chem Commun (Camb). 2021 Nov 19;57(92):12341-12344. doi: 10.1039/d1cc04787f.
We herein developed a novel method for alkaline phosphatase (ALP) assay based on the target-induced transcription of a light-up RNA aptamer, consequently producing a highly enhanced fluorescence signal upon specifically binding to the corresponding dye. Using this strategy, we successfully determined the ALP activity down to 0.018 U L (dynamic linear range of 0.04-4 U L) with excellent selectivity.
我们在此开发了一种基于亮 RNA 适体的靶标诱导转录的新型碱性磷酸酶（ALP）检测方法，当与相应染料特异性结合时，会产生高度增强的荧光信号。利用该策略，我们成功地将 ALP 活性检测下限降低至 0.018 U L（动态线性范围为 0.04-4 U L），且具有优异的选择性。
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