Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China; Guangzhou PharmaRays Technology Co., Ltd, Guangzhou, 510000, China.
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.
Virology. 2022 Jan 2;565:52-57. doi: 10.1016/j.virol.2021.10.008. Epub 2021 Oct 29.
Transposons are mobile DNAs that can move to different locations in host genomes. The integration site selection of transposons is critical for both themselves and host cells. Studies on the integration of retrotransposons and retroviruses have focused more on the global preference than on the local preference. The local preferences of retrotransposons are usually weak and of large diversity. Here, we analyzed hundreds of thousands of independent integration events of the Tf1 retrotransposon in Schizosaccharomyces pombe. The consensus sequence at the Tf1 integration sites shows a palindromic pattern, which can be divided into four sections, each of them contains one or more CGnTA units with a period of 10 base pairs, indicating interaction with subunits of the integrase oligomer in the pre-integration complex. Moreover, the analysis on the nucleosome occupancy flanking Tf1 target sites shows that Tf1 integration favors regions with one entire nucleosome depletion.
转座子是可以移动到宿主基因组中不同位置的可移动 DNA。转座子的整合位点选择对自身和宿主细胞都至关重要。关于逆转座子和逆转录病毒整合的研究更多地关注全局偏好，而不是局部偏好。逆转座子的局部偏好通常较弱且具有很大的多样性。在这里，我们分析了数以十万计的 Schizosaccharomyces pombe 中 Tf1 逆转座子的独立整合事件。Tf1 整合位点的一致序列显示出回文模式，可分为四个部分，每个部分包含一个或多个 CGnTA 单元，具有 10 个碱基对的周期，表明与整合前复合物中整合酶寡聚体的亚基相互作用。此外，对 Tf1 靶位点侧翼核小体占有率的分析表明，Tf1 整合有利于整个核小体耗竭的区域。
