Biochemistry Unit, Department of Biomedical Science, Faculty of Sciences, Rangsit University, 52/347 Muang Ake, Phaholyothin road, Lak Hok, Muang, Pathum Thani, 12000, Thailand.
Faculty of Medical Technology, Rangsit University, 52/347 Muang Ake, Phaholyothin road, Lak Hok, Muang, Pathum Thani, 12000, Thailand.
BMC Complement Med Ther. 2021 Nov 4;21(1):276. doi: 10.1186/s12906-021-03448-2.
Bauhinia strychnifolia Craib is an herb in Thai traditional medicine. Its decoction is traditionally used as an anticancer, antidiarrheal, and hangover remedy for centuries. Several studies described bioactivities of its organic solvent extracts, however, only few demonstrated the usefulness of the decoction. Here, we aimed to determine the bioactivities of Bauhinia strychnifolia Craib root and stem aqueous extracts in gut and liver perspective.
To achieve the goal, we performed MTT test, microscopic analyses, disc diffusion assay, broth microdilution assay, free radicals scavenging assays, and LC-MS analysis.
We found that the extracts inhibited the growth of human hepatocellular carcinoma (HepG2) and colon adenocarcinoma (HT-29) cell lines. Moreover, they also inhibited the growth of gram-positive bacteria Staphylococcus aureus and Bacillus cereus but not inhibited the growth of gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Furthermore, the extracts exhibited moderate antioxidant activity and increased GSH production in HepG2 cell line when compared with untreated. Our LC-MS analysis confirmed the existence of anticancer and antioxidant; 3,5,7,3',5'-pentahydroxyflavanonol-3-O-α-L-rhamnopyranoside and β-sitosterol, in the extracts.
The results from our study supported that the administration of Bauhinia strychnifolia Craib root and stem decoction would really aid colon or liver cancer patients and detoxify the alcoholic drunkard as it is claimed in Thai traditional medicine.
羊蹄甲 strychnifolia Craib 是泰国传统医学中的一种草本植物。其汤剂已被传统使用数百年，用于抗癌、止腹泻和宿醉的治疗。有几项研究描述了其有机溶剂提取物的生物活性，但只有少数研究证明了汤剂的有用性。在这里，我们旨在确定羊蹄甲 strychnifolia Craib 根和茎水提取物在肠道和肝脏方面的生物活性。
为了实现这一目标，我们进行了 MTT 试验、显微镜分析、圆盘扩散试验、肉汤微量稀释试验、自由基清除试验和 LC-MS 分析。
我们发现提取物抑制了人肝癌 (HepG2) 和结肠腺癌 (HT-29) 细胞系的生长。此外，它们还抑制了革兰氏阳性菌金黄色葡萄球菌和蜡状芽孢杆菌的生长，但不抑制革兰氏阴性菌大肠杆菌和铜绿假单胞菌的生长。此外，与未处理组相比，提取物在 HepG2 细胞系中表现出中等的抗氧化活性并增加了 GSH 的产生。我们的 LC-MS 分析证实了提取物中存在抗癌和抗氧化物质；3,5,7,3',5'-五羟基黄酮醇-3-O-α-L-鼠李吡喃糖苷和β-谷甾醇。
我们的研究结果支持泰国传统医学中所声称的，羊蹄甲 strychnifolia Craib 根和茎的汤剂给药确实可以帮助结肠癌或肝癌患者，并对酒精中毒者进行解毒。
