State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
Talanta. 2022 Jan 15;237:122960. doi: 10.1016/j.talanta.2021.122960. Epub 2021 Oct 9.
HS has been reported to play essential roles in a variety of physiological and pathological procedures. In this work, a novel fluorescent probe, Rho-HS, for detecting HS was developed by introducing the ortho-halogen to activate the least reactive recognition group 2,4-dinitrophenyl moiety. In combination of the structures from both Rhodamine B and fluorescein, Rho-HS could generate both the colorimetric and fluorescent responses. This feature was not frequently achieved and could lead to the quantitative and convenient for the end-user. In comparison with recent probes for HS, the major advantages of Rho-HS included suiting wide pH range (6.0-10.0), relatively rapid response (within 15 min) and the high selectivity among the competing species including the biothiols. With low cytoxicity, Rho-HS was further applied in the biological imaging in living MCF-7 cells and Caenorhabditis elegans. We hope that the designing strategy in this work might provide useful information for more preferable implements in this field.
HS 已被报道在多种生理和病理过程中发挥重要作用。在这项工作中，通过引入邻卤原子激活最不活泼的识别基团 2,4-二硝基苯部分，开发了一种用于检测 HS 的新型荧光探针 Rho-HS。结合 Rhodamine B 和荧光素的结构，Rho-HS 可以产生比色和荧光响应。这种特性并不常见，并且可以为最终用户提供定量和方便的检测。与最近用于 HS 的探针相比，Rho-HS 的主要优点包括适合较宽的 pH 值范围（6.0-10.0）、相对较快的响应速度（在 15 分钟内）以及在包括生物硫醇在内的竞争物种中的高选择性。由于 Rho-HS 具有低细胞毒性，因此进一步将其应用于活 MCF-7 细胞和秀丽隐杆线虫中的生物成像。我们希望这项工作中的设计策略可以为该领域提供更理想的工具提供有用的信息。
