College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.
Beijing Drainage Group Co. Ltd (BDG), Beijing 100022, China.
Bioresour Technol. 2022 Jan;344(Pt B):126267. doi: 10.1016/j.biortech.2021.126267. Epub 2021 Nov 1.
Mainstream partial-denitrification with anammox (PD-anammox) process faced the challenge of complex organics involved in real sewage. Herein, PD-anammox coupled with in-situ fermentation was successfully achieved in a full biofilm system formed by three-stage anoxic/oxic reactor to treat real wastewater with low COD/N of 3.6. The total nitrogen (TN) removal efficiency was enhanced to 78.4% ± 3.6% with average TN and ammonium concentrations in effluent of 10.6 and 0.5 mg N/L, respectively. Batch tests confirmed that partial-denitrification was the major nitrite provider for anammox in the anoxic biofilm, while in-situ fermentation could decompose the complex organics to readily-biodegradable organics for full- or partial-denitrification. Additionally, a significant anammox bacteria (Candidatus Brocadia) population was detected in the second (3.53%) and third (4.46%) anoxic zones, while denitrifiers and fermentative bacteria were mainly enriched in the first anoxic zone. This study presents a feasible approach for PD-anammox process in practical application under mainstream condition.
主流的厌氧氨氧化（PD-anammox）工艺面临着实际污水中复杂有机物的挑战。在此，通过三相缺氧/好氧反应器形成的全生物膜系统成功实现了 PD-anammox 与原位发酵的偶联，以处理 COD/N 低至 3.6 的实际废水。总氮（TN）去除效率提高到 78.4%±3.6%，出水平均 TN 和氨氮浓度分别为 10.6 和 0.5mgN/L。批处理实验证实，缺氧生物膜中的部分反硝化是厌氧氨氧化的主要亚硝酸盐提供者，而原位发酵可以将复杂有机物分解为易生物降解的有机物，用于完全或部分反硝化。此外，在第二（3.53%）和第三（4.46%）缺氧区检测到大量厌氧氨氧化菌（Candidatus Brocadia），而反硝化菌和发酵菌主要富集在第一缺氧区。本研究为主流条件下 PD-anammox 工艺在实际应用中提供了一种可行的方法。
