Department of Entomology, National Taiwan University, Taipei, 106, Taiwan.
Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH, Amsterdam, the Netherlands.
Insect Biochem Mol Biol. 2021 Dec;139:103674. doi: 10.1016/j.ibmb.2021.103674. Epub 2021 Nov 1.
Deformed wing virus (DWV) infection is believed to be closely associated with colony losses of honeybee (Apis mellifera) due to reduced learning and memory of infected bees. The adenosine (Ado) pathway is important for maintaining immunity and memory function in animals, and it enhances antivirus responses by regulating carbohydrate metabolism in insects. Nevertheless, its effect on the memory of invertebrates is not yet clear. This study investigated how the Ado pathway regulates energy metabolism and memory in honeybees following DWV infection. Decreased Ado receptor (Ado-R) expression in the brain of infected bees resulted in a carbohydrate imbalance as well as impairments of glutamate-glutamine (Glu-Gln) cycle and long-term memory. Dietary supplementation with Ado not only increased the brain energy metabolism but also rescued long-term memory loss by upregulating the expression of memory-related genes. The present study demonstrated the regulation of the Ado pathway upon DWV infection and provides insights into the mechanisms underlying energy regulation and the neurological function of honeybees.
变形翅膀病毒（DWV）感染被认为与蜜蜂（Apis mellifera）的群体损失密切相关，因为感染的蜜蜂的学习和记忆能力下降。腺苷（Ado）途径对维持动物的免疫力和记忆功能很重要，它通过调节昆虫的碳水化合物代谢来增强抗病毒反应。然而，它对无脊椎动物记忆的影响尚不清楚。本研究探讨了 DW V 感染后，Ado 途径如何调节蜜蜂的能量代谢和记忆。感染蜜蜂大脑中的 Ado 受体（Ado-R）表达减少导致碳水化合物失衡，以及谷氨酰胺-谷氨酸（Glu-Gln）循环和长期记忆受损。用 Ado 进行饮食补充不仅增加了大脑的能量代谢，还通过上调与记忆相关的基因表达来挽救长期记忆丧失。本研究证明了 Ado 途径在 DW V 感染后的调节作用，并为蜜蜂的能量调节和神经功能的机制提供了新的见解。
