Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
J Proteomics. 2022 Jan 16;251:104412. doi: 10.1016/j.jprot.2021.104412. Epub 2021 Nov 2.
The gram-negative bacterium Vibrio (Listonella) anguillarum (VA) is the causative agent of vibriosis, a terminal hemorrhagic septicemia affecting the aquacultural industry across the globe. In the current study we used label-free quantitative proteomics to investigate how VA adapts to conditions that mimic defined aspects of vibriosis-related stress such as exposure to oxidative stress (HO), exposure to humoral factors of innate immunity through incubation with Atlantic salmon serum, and iron deprivation upon supplementation of 2,2'-dipyridyl (DIP) to the growth medium. We also investigated how regulation of virulence factors may be governed by the VA growth phase and availability of nutrients. All experimental conditions explored revealed stress-specific proteomic adaption of VA and only nine proteins were found to be commonly regulated in all conditions. A general observation made for all stress-related conditions was regulation of multiple metabolic pathways. Notably, iron deprivation and exposure to Atlantic salmon serum evoked upregulation of iron acquisition mechanisms. The findings made in the present study represent a source of potential virulence determinants that can be of use in the search for means to understand vibriosis. SIGNIFICANCE: Vibriosis in fish and shellfish caused by V. anguillarum (VA) is responsible for large economic losses in the aquaculture sector across the globe. However, not much is known about the defense mechanism of this pathogen to percept and adapt to the imposed stresses during infection. Analyzing the response of VA to multiple host-related physiochemical stresses, the quantitative proteomic analysis of the present study indicates modulation of several virulence determinants and key defense networks of this pathogen. Our findings provide a theoretical basis to enhance our understanding of VA pathogenesis and can be employed to improve current intervention strategies to control vibriosis in aquaculture.
革兰氏阴性菌鳗弧菌（Listonella）anguillarum（VA）是弧菌病的病原体，弧菌病是一种影响全球水产养殖业的终末出血性败血症。在本研究中，我们使用无标记定量蛋白质组学方法研究了 VA 如何适应模拟与弧菌病相关应激相关的特定方面的条件，例如暴露于氧化应激（HO）、通过与大西洋鲑血清孵育暴露于先天免疫的体液因子以及在生长培养基中补充 2,2'-二吡啶（DIP）时缺铁。我们还研究了毒力因子的调节如何受 VA 生长阶段和营养物质可用性的控制。所有探索的实验条件都揭示了 VA 的应激特异性蛋白质组适应，只有 9 种蛋白质在所有条件下被发现共同调节。所有与应激相关的条件都观察到了多个代谢途径的调节。值得注意的是，缺铁和暴露于大西洋鲑血清引起铁摄取机制的上调。本研究的发现代表了潜在的毒力决定因素的来源，可用于寻找理解弧菌病的方法。意义：由鳗弧菌（VA）引起的鱼类和贝类弧菌病是全球水产养殖业造成巨大经济损失的原因。然而，对于这种病原体感知和适应感染过程中施加的应激的防御机制知之甚少。通过分析 VA 对多种宿主相关生理化学应激的反应，本研究的定量蛋白质组分析表明，该病原体的几种毒力决定因素和关键防御网络发生了调制。我们的研究结果为增强对 VA 发病机制的理解提供了理论基础，并可用于改进当前控制水产养殖中弧菌病的干预策略。
