Baruch Joaquin, Cernicchiaro Natalia, Cull Charley A, Lechtenberg Kelly F, Nickell Jason S, Renter David G
Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
Center for Outcomes Research and Epidemiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
Transl Anim Sci. 2021 Oct 13;5(4):txab200. doi: 10.1093/tas/txab200. eCollection 2021 Oct.
Blood leukocyte differentials can be useful for understanding changes associated with bovine respiratory disease (BRD) progression. By improving turnaround time, point-of-care leukocyte differential assays (PCLD) may provide logistical advantages to laboratory-based assays. Our objective was to assess BRD progression in steers challenged with bovine herpesvirus 1 and using point-of-care and laboratory-based blood leukocyte differentials. Thirty Holstein steers (average body weight of 211 kg 2.4 kg) were inoculated intranasally on day 0 with bovine herpesvirus 1 and intrabronchially on day 6 with . Blood leukocytes differentials were measured using both assays from study days 0 to 13. Linear mixed models were fitted to evaluate the associations between: (1) the type of assay (laboratory-based or PCLD) with respect to leukocyte, lymphocyte, and neutrophil concentrations; (2) study day with cell concentrations; and (3) cell concentrations with lung consolidation measured at necropsy. Point-of-care leukocyte, lymphocyte, and neutrophil concentrations were significantly associated ( < 0.05) with the respective cell concentrations obtained from the laboratory-based leukocyte differential. Cell concentrations reported by both assays differed significantly ( < 0.05) over time, indicating shifts from healthy to viral and bacterial disease states. Lymphocyte concentrations, lymphocyte/neutrophil ratios obtained from both assays, and band neutrophil concentrations from the laboratory-based assay were significantly associated ( < 0.05) with lung consolidation, enhancing assessments of disease severity. The PCLD may be a useful alternative to assess BRD progression when laboratory-based leukocyte differentials are impractical.
血液白细胞分类对于理解与牛呼吸道疾病（BRD）进展相关的变化可能有用。通过缩短周转时间，即时白细胞分类检测（PCLD）可能比基于实验室的检测具有后勤优势。我们的目标是使用即时检测和基于实验室的血液白细胞分类来评估感染牛疱疹病毒1的阉牛的BRD进展情况。30头荷斯坦阉牛（平均体重211千克±2.4千克）在第0天经鼻接种牛疱疹病毒1，并在第6天经支气管接种……。在研究的第0天至第13天使用这两种检测方法测量血液白细胞分类。拟合线性混合模型以评估以下各项之间的关联：（1）检测类型（基于实验室的或PCLD）与白细胞、淋巴细胞和中性粒细胞浓度的关系；（2）研究天数与细胞浓度的关系；（3）细胞浓度与尸检时肺实变的关系。即时白细胞、淋巴细胞和中性粒细胞浓度与从基于实验室的白细胞分类获得的相应细胞浓度显著相关（P<0.05）。两种检测方法报告的细胞浓度随时间有显著差异（P<0.05），表明从健康状态转变为病毒和细菌疾病状态。两种检测方法获得的淋巴细胞浓度、淋巴细胞/中性粒细胞比值以及基于实验室检测的杆状中性粒细胞浓度与肺实变显著相关（P<0.05），增强了对疾病严重程度的评估。当基于实验室的白细胞分类不切实际时，PCLD可能是评估BRD进展的一种有用替代方法。
