Zhang Ya-Ting, Cai Hao, Duan Yu, Pei Ke, Mo Zi-Qing, Niu Min-Jie, Duan Yi-Chen
School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine Nanjing 210023, China.
Institute of Chinese Medicine and Food Engineering, Shanxi University of Traditional Chinese Medicine Jinzhong 030024, China.
Zhongguo Zhong Yao Za Zhi. 2021 Oct;46(19):4993-5004. doi: 10.19540/j.cnki.cjcmm.20210319.304.
The antidepressant mechanism of Sini Powder was investigated by metabonomics based on UHPLC-Q-TOF-MS, and the roles of processing and compatibility in the antidepression of Sini Powder were discussed in the present study. The chronic unpredictable mild stress(CUMS) model of depression was induced in the model group, the Bupleuri Radix group, the Paeoniae Radix Alba group, the herb-pair group(Bupleuri Radix-Paeoniae Radix Alba), the Sini Powder group, and the vinegar-processed Sini Powder group(Bupleuri Radix and Paeoniae Radix Alba were vinegar-processed). After the establishment of the model, the rats in each group were continuously administered with corresponding drugs(ig) at a dose of 9.6 g·kg~(-1) for eight days [the rats in the model group and the normal group(without model induction) received the same volume of normal saline at the same time]. Following the last administration, the differential metabolites were identified to analyze metabolic pathways based on the rat plasma samples collected from each group. A total of sixteen potential biomarkers were identified. The metabolites with significant changes were involved in many biological metabolic pathways, such as amino acid metabolism, pentose phosphate pathway, glycerol phospholipid metabolism, sphingolipid metabolism, and purine metabolism. After drug intervention, some biomarkers returned to normal levels. Further comparisons of processing and compatibility revealed that the vinegar-processed Sini Powder group had the most total metabolic pathways where differential metabolites were returned to normal. Compared with the individual herbs, the herb-pair significantly improved the recovery of differential metabolites in the pentose phosphate and purine metabolic pathways. Compared with the Sini Powder, the vinegar-processed Sini Powder facilitated the recovery of differential metabolites in the arginine biosynthesis, and pyrimidine and pentose phosphate metabolic pathways. As indicated by the results, Sini Powder may interfere with depression by regulating lipid and nucleotide metabolisms. The processing and compatibility of Chinese herbal medicines can potentiate the intervention on depression by regulating nucleotide, energy, and amino acid metabolisms to a certain extent.
基于超高效液相色谱-四极杆飞行时间质谱联用技术的代谢组学方法研究了四逆散的抗抑郁机制，并探讨了炮制及配伍在四逆散抗抑郁作用中的作用。在模型组、柴胡组、白芍组、药对组（柴胡-白芍）、四逆散组及醋制四逆散组（柴胡和白芍醋制）中诱导建立慢性不可预测性轻度应激（CUMS）抑郁模型。造模成功后，各组大鼠以9.6 g·kg⁻¹的剂量连续灌胃相应药物8天[模型组和正常组（未造模）大鼠同时给予相同体积的生理盐水]。末次给药后，采集各组大鼠血浆样本，鉴定差异代谢物并分析代谢途径。共鉴定出16种潜在生物标志物。发生显著变化的代谢物涉及许多生物代谢途径，如氨基酸代谢、磷酸戊糖途径、甘油磷脂代谢、鞘脂代谢和嘌呤代谢。药物干预后，一些生物标志物恢复到正常水平。进一步比较炮制及配伍发现，醋制四逆散组差异代谢物恢复正常的代谢途径总数最多。与单味药相比，药对显著改善了磷酸戊糖和嘌呤代谢途径中差异代谢物的恢复情况。与四逆散相比，醋制四逆散促进了精氨酸生物合成、嘧啶和磷酸戊糖代谢途径中差异代谢物的恢复。结果表明，四逆散可能通过调节脂质和核苷酸代谢来干预抑郁症。中药的炮制及配伍可通过在一定程度上调节核苷酸、能量和氨基酸代谢来增强对抑郁症的干预作用。
