Deng Tao, Yuan Qing-Song, Zhou Tao, Guo Lan-Ping, Jiang Wei-Ke, Zhou Shi-Hua, Yang Chang-Gui, Kang Chuan-Zhi
Guizhou University of Traditional Chinese Medicine Guiyang 550025, China.
National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2021 Oct;46(20):5240-5246. doi: 10.19540/j.cnki.cjcmm.20210716.101.
Zearalenone(ZEN) is a mycotoxin produced by Fusarium, possessing estrogen-like effects, carcinogenicity, and multiple toxicities. To seek more efficient and practical agents for biological detoxification and broaden their application, this study isolated 194 bacterial strains from the moldy tuberous root of Pseudostellaria heterophylla, which were co-cultured with ZEN. An efficient ZEN-degrading strain H4-3-C1 was screened out by HPLC and identified as Acinetobacter calcoaceticus by morphological observation and molecular identification. The effects of culture medium, inoculation dose, culture time, pH, and temperature on the degradation of ZEN by H4-3-C1 strain were investigated. The mechanism of ZEN degradation and the degrading effect in Coicis Semen were discussed. The degradation rate of 5 μg·mL(-1) ZEN by H4-3-C1 strain was 85.77% in the LB medium(pH 6) at 28 ℃/180 r·min(-1) for 24 h with the inoculation dose of 1%. The degradation rate of ZEN in the supernatant of strain culture was higher than that in the intracellular fluid and thalli. The strain was inferred to secret extracellular enzymes to degrade ZEN. In addition, the H4-3-C1 strain could also degrade ZEN in Coicis Semen. If the initial content of ZEN in Coicis Semen was reduced from 90 μg·g(-1) to 40.68 μg·g(-1), the degradation rate could reach 54.80%. This study is expected to provide a new strain and application technology for the biological detoxification of ZEN in food processing products and Chinese medicinal materials.
玉米赤霉烯酮（ZEN）是一种由镰刀菌产生的霉菌毒素，具有雌激素样作用、致癌性和多种毒性。为了寻找更高效、实用的生物解毒剂并拓宽其应用范围，本研究从太子参发霉的块根中分离出194株细菌菌株，并将其与ZEN共培养。通过高效液相色谱法筛选出一株高效降解ZEN的菌株H4-3-C1，并通过形态学观察和分子鉴定将其鉴定为醋酸钙不动杆菌。研究了培养基、接种量、培养时间、pH值和温度对H4-3-C1菌株降解ZEN的影响。探讨了ZEN在薏苡仁中的降解机制及降解效果。在LB培养基（pH 6）中，28℃/180 r·min⁻¹条件下培养24 h，接种量为1%时H4-3-C1菌株对5 μg·mL⁻¹ ZEN的降解率为85.77%。菌株培养液上清中ZEN的降解率高于细胞内液和菌体中的降解率。推测该菌株通过分泌胞外酶来降解ZEN。此外，H4-3-C1菌株还能降解薏苡仁中的ZEN。若将薏苡仁中ZEN的初始含量从90 μg·g⁻¹降至40.68 μg·g⁻¹，降解率可达54.80%。本研究有望为食品加工产品和中药材中ZEN的生物解毒提供新菌株和应用技术。
