circ_0002141 通过 miR-1231/EGFR 轴促进口腔鳞状细胞癌进展。

Upregulated circ_0002141 facilitates oral squamous cell carcinoma progression via the miR-1231/EGFR axis.

机构信息

School of Management, Hainan Medical University, Haikou, Hainan, China.

School of Stomatology, Hainan Medical University, Haikou, Hainan, China.

出版信息

Oral Dis. 2023 Apr;29(3):902-912. doi: 10.1111/odi.14070. Epub 2022 Jan 18.

DOI:10.1111/odi.14070

PMID:34739167

Abstract

OBJECTIVES

The dysregulation of circular RNAs (circRNAs) is implicated in the progression of various cancers. This study was aimed at expounding the role and mechanism of hsa_circ_0002141 in the OSCC progression.

MATERIALS AND METHODS

Circ_0002141 expression was examined in 52 pairs of OSCC tissues and matched adjacent tissue samples by quantitative real-time polymerase chain reaction (qRT-PCR) assay. After circ_0002141 was overexpressed or knocked down in OSCC cell lines, cell counting kit-8 (CCK-8) assay, Transwell assay, flow cytometry, and Western blotting were conducted to detect the changes in the growth, migration, invasion and apoptosis of OSCC cells. Western blot assay, qRT-PCR and dual-luciferase reporter assay were performed to clarify the interplay among circ_0002141, miR-1231, and epidermal growth factor receptor (EGFR).

RESULTS

Circ_0002141 expression was significantly upregulated in OSCC tissues and cell lines. Circ_0002141 overexpression markedly promoted the proliferation, migration, and invasion of OSCC cells whereas reduced the apoptotic of OSCC cells. Also, circ_0002141 knockdown suppressed the malignant characteristics of OSCC cells. EGFR was validated as the target of miR-1231. Besides, circ_0002141 could sponge miR-1231 and upregulate EGFR expression in OSCC cells.

CONCLUSION

Circ_0002141/miR-1231/EGFR axis is involved in the progression of OSCC.

摘要

目的

环状 RNA（circRNAs）的失调与多种癌症的进展有关。本研究旨在阐述 hsa_circ_0002141 在口腔鳞状细胞癌（OSCC）进展中的作用和机制。

材料和方法

通过实时定量聚合酶链反应（qRT-PCR）检测 52 对 OSCC 组织和配对的相邻组织样本中的 circ_0002141 表达。在 OSCC 细胞系中转染 circ_0002141 过表达或敲低后，通过细胞计数试剂盒-8（CCK-8）检测、Transwell 检测、流式细胞术和 Western blot 检测来检测 OSCC 细胞生长、迁移、侵袭和凋亡的变化。Western blot 检测、qRT-PCR 和双荧光素酶报告基因检测用于阐明 circ_0002141、miR-1231 和表皮生长因子受体（EGFR）之间的相互作用。

结果

circ_0002141 在 OSCC 组织和细胞系中表达显著上调。circ_0002141 过表达显著促进了 OSCC 细胞的增殖、迁移和侵袭，而减少了 OSCC 细胞的凋亡。此外，circ_0002141 敲低抑制了 OSCC 细胞的恶性特征。EGFR 被验证为 miR-1231 的靶基因。此外，circ_0002141 可以海绵吸附 miR-1231 并上调 OSCC 细胞中 EGFR 的表达。