Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, No 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China.
School of Biotechnology, Jiangnan University, No 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China.
Bioprocess Biosyst Eng. 2022 Feb;45(2):425-429. doi: 10.1007/s00449-021-02660-1. Epub 2021 Nov 5.
Wastewater containing recalcitrant dyes causes environmental problems. A new superfamily of heme-containing peroxidases, dye-decolorizing peroxidases (DyPs), has been found to decolorize different kinds of dyes, especial anthraquinone dyes efficiently. However, the mechanism of dyes degradation by DyPs has not been fully understood and the toxicity of dye degradation intermediates by DyPs catalysis to microbes is unclear. In this study, a purified recombinant Thermobifida fusca DyP (TfuDyP) in E. coli BL21(DE3) was used to treat Reactive Blue 19 (RB19), an anthraquinone dye. The reaction intermediates analyzed by ultra performance liquid chromatography/mass spectroscopy (UPLC-MS) indicated the initial site of TfuDyP attack on RB19. In addition, it was found that both RB19 and its incomplete degradation products inhibited the growth of Bacillus subtilis. These findings provided a novel understanding of DyPs catalysis to anthraquinone dyes.
废水中含有难处理的染料会导致环境问题。人们发现了一种新型的含铁血红素过氧化物酶超家族，即染料脱色过氧化物酶（DyP），它可以有效地将不同种类的染料，特别是蒽醌染料脱色。然而，DyP 降解染料的机制尚未完全阐明，DyP 催化染料降解中间体对微生物的毒性也不清楚。在本研究中，使用在大肠杆菌 BL21(DE3)中表达的纯化重组嗜热真菌 DyP（TfuDyP）处理蒽醌染料活性蓝 19（RB19）。通过超高效液相色谱/质谱联用技术（UPLC-MS）分析的反应中间体表明了 TfuDyP 对 RB19 的初始攻击位点。此外，还发现 RB19 及其不完全降解产物均抑制了枯草芽孢杆菌的生长。这些发现为 DyP 催化蒽醌染料提供了新的认识。
