Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125001, Haryana, India.
Indian Council of Agricultural Research-National Research Centre on Equines, Hisar 125001, Haryana, India.
J Nanosci Nanotechnol. 2021 Jun 1;21(6):3513-3523. doi: 10.1166/jnn.2021.19000.
Bluetongue (BT) disease is a noncontagious disease of domestic and wild ruminants (mainly sheep, cattle, deer) caused by the bluetongue virus (BTV) which is an orbivirus of the Reoviridae family and transmitted by vector Culicoides biting midges. It is a reportable disease of considerable socioeconomic concern and of major importance for the international trade of animals and animal products. Conventional diagnostic methods, such as virus propagation and isolation, immunoassays and also various molecular methods have been developed for the detection of the BTV. Here, we present a novel, rapid and pen-side test for the detection of BTV using multiwalled carbon nanotube (MWCNTs) based immunosensor. Though it is not reported yet. The MWCNTs were prepared, characterized and functionalized with carboxyl group. Viral antibodies were conjugated successfully with functionalized MWCNTs and coated on screen printed carbon electrode (SPCE). These SPCE were evaluated by using electrochemical sensor with an antigen specific to BTV antibodies, resulted in the self-assembled layer of antigen-antibody on the surface of SPCE. The approach described in the present study is a prototype for the development of simple and economic diagnostic tool which will provide the routine screening of BT disease at the door of farmers, thereby increasing the income of farmers by decreasing the cost of diagnosis.
蓝舌病是一种由蓝舌病毒（BTV）引起的非传染性家畜和野生动物（主要是绵羊、牛、鹿）疾病，该病毒是呼肠孤病毒科的环状病毒，由媒介蠓传播。它是一种具有相当社会经济意义的报告疾病，对动物和动物产品的国际贸易具有重要意义。已经开发了常规诊断方法，例如病毒繁殖和分离、免疫测定以及各种分子方法，用于检测 BTV。在这里，我们使用基于多壁碳纳米管 (MWCNT) 的免疫传感器展示了一种用于检测 BTV 的新型快速笔侧检测方法。尽管尚未报道。MWCNTs 经过制备、表征和羧基功能化。病毒抗体成功地与功能化的 MWCNTs 共轭，并涂覆在丝网印刷碳电极 (SPCE) 上。这些 SPCE 通过使用针对 BTV 抗体的抗原的电化学传感器进行了评估，导致抗原-抗体在 SPCE 表面的自组装层。本研究中描述的方法是开发简单经济的诊断工具的原型，该工具将在农民家门口提供 BT 病的常规筛查，从而通过降低诊断成本来增加农民的收入。
