Department of Bio & Nanotechnology, Guru Jambheshwar University of Science & Technology, Hisar 125001, Haryana, India.
Faculty of Agricultural Sciences, SGT University, Gurugram 122006, Haryana, India.
J Nanosci Nanotechnol. 2021 Jun 1;21(6):3531-3538. doi: 10.1166/jnn.2021.19010.
Cotton ( L.), is an important fibre and oilseed crop of the world. India in particular has the largest area under cotton cultivation and around 60% proportion in the raw fibre textile industry is contributed by cotton alone. Cotton is affected by many diseases (bacterial blights, fungal leafspots, mildew) and pests (white flies, bollworms, aphids etc.). The bacterial blight disease caused by pv. is considered as one of the most devastating one that cause huge losses in production every year. Due to systemic spread of this bacterial infection, combating this disease is slightly challenging. Spray of toxic chemicals like endosulfan, streptocycline and dimethoate is a common practice in fields but these chemicals are unable to control the disease spread substantially. Nanotechnology is a newly emerging technology that is being extensively exploited in the agriculture sector these days. Past studies have reported the antimicrobial effect of various metallic nanoparticles including zinc oxide nanoparticles which is known to possess antibacterial potential against both gram +ve and gram -ve bacteria. Based upon this, synthesis of ZnO nanoparticles was carried out using plant leaf extract and the nanoparticles were characterised in detail using scanning electron microscopy, atomic force microscopy, X-ray diffraction analysis, electron dispersive X-ray spectroscopy study etc. The zinc oxide nanoparticles were found crystalline in nature and the size ranged between 10-50 nanometers. The efficacy of these nanoparticles was checked against pv. under conditions and found to be very effective in controlling the bacterial spread in comparison to streptomycin that was used as control. Our results suggest that ZnO nanoparticles can be used as an effective antibacterial agent to control bacterial blight disease of cotton.
棉花（L.）是世界上一种重要的纤维和油料作物。特别是印度，其棉花种植面积最大，棉花在原纤维纺织工业中的比例约为 60%。棉花受到许多疾病（细菌枯萎病、真菌叶斑病、霉病）和害虫（粉虱、棉铃虫、蚜虫等）的影响。由 pv. 引起的细菌性枯萎病被认为是最具破坏性的疾病之一，每年都会造成巨大的产量损失。由于这种细菌性感染的系统性传播，防治这种疾病有点具有挑战性。在田间喷洒像硫丹、链霉素和乐果等有毒化学物质是一种常见的做法，但这些化学物质无法有效地控制疾病的传播。纳米技术是一种新兴的技术，目前在农业领域得到了广泛的应用。过去的研究报告了各种金属纳米粒子的抗菌作用，包括氧化锌纳米粒子，它被认为具有抗革兰氏阳性和革兰氏阴性细菌的抗菌潜力。基于此，使用植物叶片提取物合成了氧化锌纳米粒子，并使用扫描电子显微镜、原子力显微镜、X 射线衍射分析、电子能谱研究等对纳米粒子进行了详细的表征。氧化锌纳米粒子为结晶态，尺寸在 10-50 纳米之间。在条件下，这些纳米粒子的功效被检查了对 pv. 的作用，结果发现与用作对照的链霉素相比，它们在控制细菌传播方面非常有效。我们的结果表明，氧化锌纳米粒子可用作有效的抗菌剂来控制棉花的细菌性枯萎病。
