State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Department of Plant pathology, Minia University, Elminya 61519, Egypt.
Molecules. 2020 Oct 19;25(20):4795. doi: 10.3390/molecules25204795.
Bacterial leaf blight caused by pv. (Xoo) is one of the most devastating diseases, resulting in significant yield losses in rice. The extensive use of chemical antibacterial agents has led to an increase the environmental toxicity. Nanotechnology products are being developed as a promising alternative to control plant disease with low environmental impact. In the present study, we investigated the antibacterial activity of biosynthesized chitosan nanoparticles (CSNPs) and zinc oxide nanoparticles (ZnONPs) against rice pathogen Xoo. The formation of CSNPs and ZnONPs in the reaction mixture was confirmed by using UV-vis spectroscopy at 300-550 nm. Moreover, CSNPs and ZnONPs with strong antibacterial activity against were further characterized by scanning and transmission electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction. Compared with the corresponding chitosan and ZnO alone, CSNPs and ZnONPs showed greater inhibition in the growth of Xoo, which may be mainly attributed to the reduction in biofilm formation and swimming, cell membrane damage, reactive oxygen species production, and apoptosis of bacterial cells. Overall, this study revealed that the two biosynthesized nanoparticles, particularly CSNPs, are a promising alternative to control rice bacterial disease.
由 pv. (Xoo)引起的细菌性叶斑病是最具破坏性的疾病之一，导致水稻产量大幅下降。抗菌化学制剂的广泛使用导致了环境毒性的增加。纳米技术产品作为一种具有低环境影响的控制植物病害的有前途的替代方法正在被开发。在本研究中，我们研究了生物合成壳聚糖纳米颗粒 (CSNPs) 和氧化锌纳米颗粒 (ZnONPs) 对水稻病原体 Xoo 的抗菌活性。通过在 300-550nm 处使用紫外-可见光谱证实了反应混合物中 CSNPs 和 ZnONPs 的形成。此外，具有强抗菌活性的 CSNPs 和 ZnONPs 进一步通过扫描和透射电子显微镜、傅里叶变换红外光谱和 X 射线衍射进行了表征。与相应的壳聚糖和 ZnO 相比，CSNPs 和 ZnONPs 对 Xoo 的生长表现出更大的抑制作用，这可能主要归因于生物膜形成和泳动的减少、细胞膜损伤、活性氧物质的产生和细菌细胞的凋亡。总的来说，这项研究表明，这两种生物合成的纳米颗粒，特别是 CSNPs，是一种有前途的控制水稻细菌性病害的替代方法。
