Department of Seed Science and Technology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India.
Centre for Bio-Nanotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India.
J Nanosci Nanotechnol. 2021 Jun 1;21(6):3566-3572. doi: 10.1166/jnn.2021.19013.
Wide range of nanomaterial applications is helpful to promote sustainable agriculture to the point of nanolevel. With regard to this, biocompatible silica nanoparticles have been used as a nanopriming agent for enhancing seed germination in rabi crops like wheat, pea and mustard. The current study was carried out in a completely randomized block design with four replications. One part of nanosilica (8 g/L) and three parts of Gibberellic acid (GA₃) loaded nano-silica (100 ppm, 125 ppm and 150 ppm) were used for the experimental purpose. The result showed positive significant effect of nanopriming with nano-silica and GA3 loaded nano-silica on seed germination percentage, shoot and root length, seedling length, fresh weight, dry weight and vigour index-I and II at ≤ 0.05. A comparative study on germination percentage for three of the cereal crops was done and calculated further. It was found to be higher as 91% in wheat, 91% in pea and 75% in mustard. This effect of nanopriming directly help in elevating the activity of -amylase enzyme, resulting in higher concentration of soluble sugar content needed for supporting seed germination and growth of seedling. In this paper, the nanopriming-induced seed germination is proposed, including the creation of nanopores for enhanced water uptake, rebooting ROS/antioxidant systems in seeds, generation of hydroxyl radicals for cell wall loosening, and nano-catalyst to fasten starch hydrolysis.
纳米材料的广泛应用有助于将可持续农业提升到纳米水平。在此背景下,生物相容性二氧化硅纳米颗粒已被用作纳米引发剂,以提高冬小麦、豌豆和芥菜等冬季作物的种子发芽率。本研究采用完全随机区组设计,设 4 次重复。实验部分使用了 8 g/L 的纳米硅和 3 部分负载赤霉素(GA₃)的纳米硅(100 ppm、125 ppm 和 150 ppm)。结果表明,纳米硅和负载 GA₃的纳米硅的纳米引发对种子发芽率、芽和根长、幼苗长度、鲜重、干重和活力指数-I 和 II 有显著的积极影响,差异均达到 0.05 水平。对三种谷物作物的发芽率进行了比较研究,并进一步计算。结果发现,小麦的发芽率最高,为 91%,豌豆为 91%,芥菜为 75%。纳米引发的这种作用直接有助于提高 -淀粉酶酶的活性,从而产生更高浓度的可溶性糖,以支持种子发芽和幼苗生长。本文提出了纳米引发的种子发芽机制,包括为增强水分吸收而创造纳米孔、重新启动种子中 ROS/抗氧化系统、产生用于细胞壁疏松的羟基自由基,以及纳米催化剂来加速淀粉水解。