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介孔纳米二氧化硅(SiO₂)对小麦、豌豆和芥菜种子萌发和幼苗生长的影响。

Impact of Mesoporous Nano-Silica (SiO₂) on Seed Germination and Seedling Growth of Wheat, Pea and Mustard Seed.

机构信息

Department of Seed Science and Technology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India.

Centre for Bio-Nanotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India.

出版信息

J Nanosci Nanotechnol. 2021 Jun 1;21(6):3566-3572. doi: 10.1166/jnn.2021.19013.

Abstract

Wide range of nanomaterial applications is helpful to promote sustainable agriculture to the point of nanolevel. With regard to this, biocompatible silica nanoparticles have been used as a nanopriming agent for enhancing seed germination in rabi crops like wheat, pea and mustard. The current study was carried out in a completely randomized block design with four replications. One part of nanosilica (8 g/L) and three parts of Gibberellic acid (GA₃) loaded nano-silica (100 ppm, 125 ppm and 150 ppm) were used for the experimental purpose. The result showed positive significant effect of nanopriming with nano-silica and GA3 loaded nano-silica on seed germination percentage, shoot and root length, seedling length, fresh weight, dry weight and vigour index-I and II at ≤ 0.05. A comparative study on germination percentage for three of the cereal crops was done and calculated further. It was found to be higher as 91% in wheat, 91% in pea and 75% in mustard. This effect of nanopriming directly help in elevating the activity of -amylase enzyme, resulting in higher concentration of soluble sugar content needed for supporting seed germination and growth of seedling. In this paper, the nanopriming-induced seed germination is proposed, including the creation of nanopores for enhanced water uptake, rebooting ROS/antioxidant systems in seeds, generation of hydroxyl radicals for cell wall loosening, and nano-catalyst to fasten starch hydrolysis.

摘要

纳米材料的广泛应用有助于将可持续农业提升到纳米水平。在此背景下,生物相容性二氧化硅纳米颗粒已被用作纳米引发剂,以提高冬小麦、豌豆和芥菜等冬季作物的种子发芽率。本研究采用完全随机区组设计,设 4 次重复。实验部分使用了 8 g/L 的纳米硅和 3 部分负载赤霉素(GA₃)的纳米硅(100 ppm、125 ppm 和 150 ppm)。结果表明,纳米硅和负载 GA₃的纳米硅的纳米引发对种子发芽率、芽和根长、幼苗长度、鲜重、干重和活力指数-I 和 II 有显著的积极影响,差异均达到 0.05 水平。对三种谷物作物的发芽率进行了比较研究,并进一步计算。结果发现,小麦的发芽率最高,为 91%,豌豆为 91%,芥菜为 75%。纳米引发的这种作用直接有助于提高 -淀粉酶酶的活性,从而产生更高浓度的可溶性糖,以支持种子发芽和幼苗生长。本文提出了纳米引发的种子发芽机制,包括为增强水分吸收而创造纳米孔、重新启动种子中 ROS/抗氧化系统、产生用于细胞壁疏松的羟基自由基,以及纳米催化剂来加速淀粉水解。

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