BioMol-Lab, Campus do Pici, Universidade Federal do Ceará, Fortaleza, Ceará 60.440-970, Brazil.
BioMol-Lab, Campus do Pici, Universidade Federal do Ceará, Fortaleza, Ceará 60.440-970, Brazil; Laboratory for Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):1562-1571. doi: 10.1016/j.ijbiomac.2021.10.219. Epub 2021 Nov 3.
Lectins from plants of the Diocleinae subtribe often exhibit specificity towards mannose/glucose and derived sugars, with some plants also displaying a second lectin specific to lactose/GalNAc. Here, we present a novel lectin from Collaea speciosa, named CsL, that displays specificity for GlcNAc/glucose. The lectin was extracted from Collaea speciosa seeds and purified by a single chromatographic step on a Sephadex G-50 matrix. In solution, the lectin appears as a dimeric protein composed of 25 kDa monomers. The protein is stable at pH 7-8 and dependent on divalent cations. CsL maintained its agglutination activity after heating to 90 °C for 1 h. Glycan array studies revealed that CsL binds to N-glycans with terminal GlcNAc residues, chitobiose and chitotriose moieties. The partial amino acid sequence of the lectin is similar to that of some lactose-specific lectins from the same subtribe. In contrast to other ConA-like lectins, CsL is not toxic to Artemia. Because of its remarkably different properties and specificity, this lectin could be the first member of a new group inside the Diocleinae lectins.
Diocleinae 亚族植物来源的凝集素通常对甘露糖/葡萄糖及其衍生糖具有特异性，有些植物还显示出第二种对乳糖/半乳糖 N-乙酰基的特异性凝集素。在这里，我们介绍了一种来自 Collaea speciosa 的新型凝集素，命名为 CsL，它对 GlcNAc/葡萄糖具有特异性。该凝集素从 Collaea speciosa 种子中提取，并通过在 Sephadex G-50 基质上进行单一色谱步骤进行纯化。在溶液中，该凝集素呈现为由 25 kDa 单体组成的二聚体蛋白。该蛋白在 pH 7-8 下稳定且依赖于二价阳离子。CsL 在加热至 90°C 1 小时后仍保持其凝集活性。糖芯片研究表明，CsL 与具有末端 GlcNAc 残基、壳二糖和壳三糖部分的 N-聚糖结合。该凝集素的部分氨基酸序列与来自同一亚族的一些乳糖特异性凝集素有相似之处。与其他 ConA 样凝集素不同，CsL 对卤虫没有毒性。由于其性质和特异性明显不同，这种凝集素可能是 Diocleinae 凝集素中一个新的凝集素家族的第一个成员。
