Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
Livestock Production and Management Section, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
Top Companion Anim Med. 2022 Jan-Feb;46:100612. doi: 10.1016/j.tcam.2021.100612. Epub 2021 Nov 3.
The objective of the study was to ascertain the role of prostaglandins Viz., PGE and PGF, and their respective receptors in the pathophysiology of canine pyometra. Normal (n = 6) and pyometra (n = 8) affected uterus were collected from bitches undergoing ovariohysterectomy. Pyometra was graded according to histopathological alterations. The levels of PGE and PGF were estimated in the endometrium. The differential expression in the mRNA of PGF receptor (FP) and PGE receptors (EP1, EP2, EP3, and EP4) were studied in the endometrium and myometrium of the pyometra-affected uterus. Normal uterus served as calibrator. Elevation of both PGE and PGF levels in the endometrium of pyometra-affected bitches was observed. The FP receptor gene in the endometrium and myometrium of pyometra-affected bitches was downregulated (P < .05). Out of all EP receptors, only EP2 receptor has shown upregulation in both endometrium and myometrium of pyometra affected uterus. EP3 receptor got downregulated in both endometrium and myometrium in pyometra. Thus, downregulation of FP, EP3 receptors in the myometrium reinforces the lack of contractility in pyometra-affected bitches favoring bacterial proliferation and subsequent pus accumulation. Moreover, upregulation of EP2 receptors in the pyometra bitches suggests the scope of selective pharmacological inhibition of EP2 receptors as an adjunct therapy in the treatment of pyometra.
本研究旨在确定前列腺素（如 PGE 和 PGF）及其各自受体在犬子宫蓄脓的病理生理学中的作用。从接受卵巢子宫切除术的母犬中收集正常（n=6）和子宫蓄脓（n=8）受影响的子宫。根据组织病理学改变对子宫蓄脓进行分级。在内膜中估计 PGE 和 PGF 的水平。研究了受影响子宫的子宫内膜和子宫肌层中 PGF 受体（FP）和 PGE 受体（EP1、EP2、EP3 和 EP4）的 mRNA 差异表达。正常子宫作为校准器。观察到患有子宫蓄脓的母犬子宫内膜中 PGE 和 PGF 水平升高。患有子宫蓄脓的母犬子宫内膜和子宫肌层中的 FP 受体基因下调（P<.05）。在所有 EP 受体中，只有 EP2 受体在受影响的子宫蓄脓的子宫内膜和子宫肌层中上调。EP3 受体在子宫蓄脓的子宫内膜和子宫肌层中下调。因此，FP、EP3 受体在子宫肌层中的下调加强了子宫蓄脓母犬的缺乏收缩性，有利于细菌增殖和随后的脓液积聚。此外，EP2 受体在子宫蓄脓母犬中的上调表明选择性抑制 EP2 受体的药理学抑制作为子宫蓄脓治疗的辅助治疗的范围。
