Wiemann M C, Dexter D L, Calabresi P
J Natl Cancer Inst. 1987 Jul;79(1):93-102.
The morphological characteristics and the production of biochemical markers were determined for 8 human tumor cell lines grown in artificial capillary culture. Comparisons were made with nude mouse xenografts and conventional monolayer or suspension cultures. Capillary histologies reproduced the features of neoplastic differentiation and glandular formation exhibited by the original human tumors and xenografts. The concentrations of specific biochemical markers, such as carcinoembryonic antigen, aspartate aminotransferase, and immunoglobulin, were higher in the pericellular culture medium in capillary culture. The capillary environment influenced the expression of biochemical heterogeneity by the DLD-1 colon carcinoma cell line and its derivative clones. Spontaneous differentiation of K562 leukemia cells was increased in the capillary system. These results indicate that the artificial capillary is a useful and relevant system for the study of cultured human tumor cells.
对在人工毛细管培养中生长的8种人类肿瘤细胞系的形态特征和生化标志物产生情况进行了测定。并与裸鼠异种移植瘤以及传统的单层或悬浮培养进行了比较。毛细管组织学再现了原始人类肿瘤和异种移植瘤所表现出的肿瘤分化和腺形成特征。在毛细管培养中，细胞周培养基中癌胚抗原、天冬氨酸转氨酶和免疫球蛋白等特定生化标志物的浓度更高。毛细管环境影响了DLD-1结肠癌细胞系及其衍生克隆的生化异质性表达。在毛细管系统中，K562白血病细胞的自发分化增加。这些结果表明，人工毛细管是研究培养的人类肿瘤细胞的一个有用且相关的系统。
