Chen Lin, Yun Maomao, Cao Zhenqiang, Liang Zhaojun, Liu Wenrui, Wang Min, Yan Jinqiang, Yang Songguang, He Xiaoming, Jiang Biao, Peng Qingwu, Lin Yu'e
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou, China.
Front Plant Sci. 2021 Oct 22;12:758976. doi: 10.3389/fpls.2021.758976. eCollection 2021.
Cucumber ( L.) is an important vegetable crop, which is thermophilic not heat resistant. High-temperature stress always results in sterility at reproductive stage. In the present study, we evaluate the male flower developmental changes under normal (CK) and heat stress (HS) condition. After HS, the activities of peroxidase (POD) and superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) were increased. In addition, the pollen fertility was significantly decreased; and abnormal tapetum and microspore were observed by paraffin section. Transcriptome analysis results presented that total of 5828 differentially expressed genes (DEGs) were identified after HS. Among these DEGs, 20 DEGs were found at four stages, including DNA binding transcription factor, glycosyltransferase, and wound-responsive family protein. The gene ontology term of carbohydrate metabolic process was significantly enriched in all anther stages, and many saccharides and starch synthase-related genes, such as invertase, sucrose synthase, and starch branching enzyme, were significantly different expressed in HS compared with CK. Furthermore, co-expression network analysis showed a module (midnightblue) strongly consistent with HS, and two hub genes ( and ) were found with a high degree of connectivity to other genes. Our results provide comprehensive understandings on male flower development in cucumber under HS.
黄瓜（L.）是一种重要的蔬菜作物，喜温但不耐热。高温胁迫总是导致生殖阶段不育。在本研究中，我们评估了正常（CK）和热胁迫（HS）条件下雄花发育的变化。热胁迫后，过氧化物酶（POD）和超氧化物歧化酶（SOD）的活性以及丙二醛（MDA）的含量增加。此外，花粉育性显著降低；通过石蜡切片观察到绒毡层和小孢子异常。转录组分析结果表明，热胁迫后共鉴定出5828个差异表达基因（DEG）。在这些DEG中，在四个阶段发现了20个DEG，包括DNA结合转录因子、糖基转移酶和伤口响应家族蛋白。碳水化合物代谢过程的基因本体术语在所有花药阶段均显著富集，与对照相比，许多糖类和淀粉合酶相关基因，如转化酶、蔗糖合酶和淀粉分支酶，在热胁迫下差异显著表达。此外，共表达网络分析显示一个模块（午夜蓝）与热胁迫高度一致，并且发现两个中心基因（和）与其他基因具有高度的连接性。我们的结果为热胁迫下黄瓜雄花发育提供了全面的认识。
