Second Ward of Breast Surgery, Shanxi Cancer Hospital, TaiYuan, China.
Third Ward of Thoracic Surgery, Shanxi Cancer Hospital, TaiYuan, China.
Bioengineered. 2021 Dec;12(2):10117-10125. doi: 10.1080/21655979.2021.2000733.
In view of the role of miR-138 in cancer cells, we predicted the target of miR-138 and its targeting to SEMA4C by bioinformatics software and luciferase experiment. The expression levels of miR-138 in human normal breast epithelial cells and two kinds of BC cells were compared, and the transfection cells were selected. MiR-138 mimetic negative control (miR-NC), miR-138 mimic and miR-138 inhibitor were designed for cell transfection. The results showed that the expression level of miR-138 in MCF-7 cells was the lowest. The up regulation of miR-138 would lead to the high expression of E-cad and the low expression of N-cad, vim and SEMA4C, and the vitality and invasion of BC cells would decrease. The down regulation of miR-138 would lead to the low expression of E-cad and the high expression of N-cad, vim and SEMA4C, and the vitality and invasion of BC cells would increase. miR-138 targeted regulation of SEMA4C can promote the expression of N-cad, inhibit the expression of E-cad, vim and SEMA4C, reverse the EMT of BC cells, and inhibit the activity and invasion of BC cells. MiR-138 has clinical potential as a tumor marker of BC.
鉴于 miR-138 在癌细胞中的作用，我们通过生物信息学软件和荧光素酶实验预测了 miR-138 的靶标及其对 SEMA4C 的靶向作用。比较了 miR-138 在人正常乳腺上皮细胞和两种 BC 细胞中的表达水平，并选择了转染细胞。设计了 miR-138 模拟物阴性对照（miR-NC）、miR-138 模拟物和 miR-138 抑制剂进行细胞转染。结果表明，MCF-7 细胞中 miR-138 的表达水平最低。miR-138 的上调会导致 E-cad 的高表达和 N-cad、vim 和 SEMA4C 的低表达，BC 细胞的活力和侵袭性降低。miR-138 的下调会导致 E-cad 的低表达和 N-cad、vim 和 SEMA4C 的高表达，BC 细胞的活力和侵袭性增加。miR-138 对 SEMA4C 的靶向调节可以促进 N-cad 的表达，抑制 E-cad、vim 和 SEMA4C 的表达，逆转 BC 细胞的 EMT，并抑制 BC 细胞的活性和侵袭性。miR-138 具有作为 BC 肿瘤标志物的临床潜力。
