Department of Medicine, Lady Hardinge Medical College and Associated Hospitals, Delhi, India.
Department of Neurology, Lady Hardinge Medical College and Associated Hospitals, Delhi, India.
Neurol India. 2021 Sep-Oct;69(5):1217-1221. doi: 10.4103/0028-3886.329586.
Stroke is one of the leading causes of death and disability in India. Angiotensin-converting enzyme (ACE) is involved in the development of hypertension, atherosclerosis, cardio, and cerebrovascular disease and is a plausible genetic candidate for stroke. The role of ACE polymorphism is still uncertain and requires further investigation.
The aim of this study was to study the role of ACE insertion/deletion polymorphism in acute ischemic stroke (AIS).
One hundred thirty AIS cases and 130 age and sex matched healthy controls were recruited. Ten milliliters venous blood was drawn and 6 mL of blood was used for routine hematological, biochemical tests. Remaining 4 mL of EDTA blood was used for DNA extraction, PCR amplification, and restriction digestion. Three genotypes (II, ID, and DD) were visualized on 3% agarose gel. Association between genotypes among stroke case was done by Chi-square test with P value <0.05 taken as significant.
DD genotype was significantly associated with the risk of stroke with P value of 0.0001. Both the dominant and recessive models showed that the DD genotype was independently associated with an increased risk of ischemic stroke (OR = 20.732; 95% CI: 2.7241-157.7864; P value = 0.003 for the recessive model and OR = 2.848; 95% CI: 1.5127-5.3649; P value = 0.001 for dominant model).
Our study showed a strong association between ACE polymorphism and the risk of AIS. This study paves the way for further studies to confirm the role of ACE polymorphism as genetic risk factors for AIS.
中风是印度导致死亡和残疾的主要原因之一。血管紧张素转换酶（ACE）参与高血压、动脉粥样硬化、心脏和脑血管疾病的发展，是中风的合理遗传候选物。ACE 多态性的作用尚不确定，需要进一步研究。
本研究旨在探讨 ACE 插入/缺失多态性在急性缺血性中风（AIS）中的作用。
招募了 130 例 AIS 病例和 130 例年龄和性别匹配的健康对照者。抽取 10 毫升静脉血，其中 6 毫升用于常规血液生化检查。剩余 4 毫升 EDTA 血用于 DNA 提取、PCR 扩增和限制性消化。在 3%琼脂糖凝胶上观察到三种基因型（II、ID 和 DD）。采用卡方检验分析基因型与中风病例的关系，P 值<0.05 为有统计学意义。
DD 基因型与中风风险显著相关，P 值为 0.0001。显性和隐性模型均表明，DD 基因型与缺血性中风风险增加独立相关（OR=20.732；95%CI：2.7241-157.7864；P 值=0.003 对于隐性模型和 OR=2.848；95%CI：1.5127-5.3649；P 值=0.001 对于显性模型）。
本研究表明 ACE 多态性与 AIS 风险之间存在很强的关联。本研究为进一步研究 ACE 多态性作为 AIS 的遗传危险因素奠定了基础。
