采用 EUCAST 9.3.2 方法对烟曲霉广义种进行分光光度唑类药物和两性霉素 B MIC 读数。是否 90%和 95%真菌生长抑制终点同样适用？
Spectrophotometric azole and amphotericin B MIC readings against Aspergillus fumigatus sensu lato using the EUCAST 9.3.2 methodology. Are ≥90 and ≥95% fungal growth inhibition endpoints equally suitable?
机构信息
Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain.
Instituto de Investigación Sanitaria Gregorio Marañón, 28007 Madrid, Spain.
出版信息
Med Mycol. 2021 Dec 8;60(1). doi: 10.1093/mmy/myab072.
UNLABELLED
We recently reported high essential (97.1%) and categorical (99.6%) agreements between azole and amphotericin B MICs against Aspergillus fumigatus sensu lato obtained by visual and spectrophotometric readings using a ≥ 95% fungal growth endpoint and following the EUCAST methodology (doi: 10.1128/AAC.01693-20). Here, we compared the aforementioned MICs against spectrophotometric MIC readings obtained using a ≥ 90% inhibition endpoint. Spectrophotometric readings using either ≥ 90% or ≥ 95% fungal growth inhibition resulted in high categorical (>99.9%) agreements with visual MIC readings against A. fumigatus sensu stricto. In contrast, agreements with visual MICs against cryptic species were higher with the use of a ≥ 95% fungal growth inhibition endpoint.
LAY SUMMARY
Spectrophotometrically obtained MIC readings using either ≥ 90% or ≥ 95% fungal growth inhibition endpoints and following the EUCAST methodology are suitable against A. fumigatus sensu stricto. However, the ≥ 95% fungal growth inhibition endpoint is preferred against cryptic species.
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我们最近报道了使用 ≥95%真菌生长终点和 EUCAST 方法（doi：10.1128/AAC.01693-20）通过目测和分光光度读数获得的针对烟曲霉广义种的唑类和两性霉素 B MIC 具有很高的一致性（97.1%）和分类一致性（99.6%）。在这里，我们比较了上述 MIC 与使用 ≥90%抑制终点获得的分光光度 MIC 读数。使用 ≥90%或 ≥95%的真菌生长抑制的分光光度读数与针对烟曲霉狭义种的目测 MIC 读数具有很高的分类一致性（>99.9%）。相比之下，使用 ≥95%的真菌生长抑制终点与隐种的目测 MIC 具有更高的一致性。
摘要
使用 ≥90%或 ≥95%的真菌生长抑制终点并遵循 EUCAST 方法获得的分光光度 MIC 读数适用于烟曲霉狭义种。然而，≥95%的真菌生长抑制终点更适用于隐种。
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