Exercise Cell Biology Lab, Faculty of Applied Sciences, University of Campinas, Limeira, Brazil; Laboratory of Molecular Biology of Exercise, Faculty of Applied Sciences, University of Campinas, Limeira, Brazil.
Laboratory of Nutritional Genomics, Faculty of Applied Sciences, University of Campinas, Limeira, Brazil.
Life Sci. 2021 Dec 15;287:120124. doi: 10.1016/j.lfs.2021.120124. Epub 2021 Nov 6.
Hepatic steatosis is directly associated with hepatic inflammation and insulin resistance, which is correlated with hyperglycemia and type 2 diabetes mellitus (T2DM). Aerobic and strength training have been pointed out as efficient strategies against hepatic steatosis. However, little is known about the effects of the combination of those two protocols on hepatic steatosis. Therefore, this study aimed to evaluate the impact of short-term combined training (STCT) on glucose homeostasis and in the synthesis and oxidation of fat in the liver of obesity-induced mice with hepatic steatosis. Swiss mice were distributed into three groups: control lean (CTL), sedentary obese (OB), and combined training obese (CTO). The CTO group performed the STCT protocol, which consisted of strength and aerobic exercises in the same session. The protocol lasted seven days. The CTO group reduced the glucose levels and fatty liver when compared to the OB group. Interestingly, these results were observed even without reductions in body adiposity. CTO group also showed increased hepatic insulin sensitivity, with lower hepatic glucose production (HGP). STCT reduced the expression of the lipogenic genes Fasn and Scd1 and hepatic inflammation, as well as increased the ACC phosphorylation and the oxidative genes Cpt1a and Ppara, reverting the complications caused by obesity. Since this protocol increased lipid oxidation and reduced hepatic lipogenesis, regardless of body fat mass decrease, it can be considered an effective non-pharmacological strategy for the treatment of hepatic steatosis.
肝脂肪变性与肝炎症和胰岛素抵抗直接相关，而后者与高血糖和 2 型糖尿病（T2DM）相关。有氧运动和力量训练已被指出是对抗肝脂肪变性的有效策略。然而，对于这两种方案的联合应用对肝脂肪变性的影响，人们知之甚少。因此，本研究旨在评估短期联合训练（STCT）对肥胖诱导的肝脂肪变性小鼠葡萄糖稳态和肝脏脂肪合成和氧化的影响。瑞士小鼠被分为三组：对照瘦（CTL）、久坐肥胖（OB）和联合训练肥胖（CTO）。CTO 组进行了 STCT 方案，即力量和有氧运动在同一时间段进行。该方案持续了七天。与 OB 组相比，CTO 组降低了血糖水平和脂肪肝。有趣的是，即使没有降低体脂，这些结果也观察到了。CTO 组还表现出更高的肝胰岛素敏感性，肝葡萄糖生成（HGP）更低。STCT 降低了脂肪生成基因 Fasn 和 Scd1 的表达以及肝炎症，同时增加了 ACC 磷酸化和氧化基因 Cpt1a 和 Ppara，从而逆转了肥胖引起的并发症。由于该方案增加了脂质氧化并减少了肝脂肪生成，而与体脂肪量的减少无关，因此它可以被认为是治疗肝脂肪变性的一种有效的非药物策略。
