Food and Agricultural Sciences, Fukushima University, Fukushima, Japan.
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
J Nutr Biochem. 2022 Feb;100:108897. doi: 10.1016/j.jnutbio.2021.108897. Epub 2021 Nov 5.
Zebrafish models have been developed for several studies involving lipid metabolism and lipid-related diseases. In the present study, the migration of dietary docosahexaenoic acid (DHA) in whole-body zebrafish was estimated by stable-isotope tracer and matrix-assisted laser desorption/ionization mass spectrometry imaging. Administration of 1-C-2,2-D-labeled DHA ((+3)DHA) ethyl ester to male zebrafish was conducted to evaluate its accumulation, migration, and distribution in the body. The (+3)DHA content in the body of zebrafish after administering (+3)DHA for 10 and 15 d was significantly higher than that in the control group. (+3)DHA was observed as a constituent of phosphatidylcholine (PC) in the intestine of zebrafish that were administered (+3)DHA for 5 and 10 d. (+3)DHA-containing PC tended to accumulate in the intestines of zebrafish administered (+3)DHA for 1 d, indicating that recombination of (+3)DHA from ethyl ester to PC occurs quickly at intestine. After administration for 15 d, (+3)DHA-containing PC accumulated in the intestine, liver, and muscle of whole-body zebrafish. In contrast, (+3)DHA-containing PC was not detected in the brain. These results showed that dietary DHA is initially constructed into PC as a structural component of intestinal cell membranes and gradually migrates into peripheral tissues such as muscle.
斑马鱼模型已被开发用于涉及脂质代谢和脂质相关疾病的多项研究。在本研究中，通过稳定同位素示踪和基质辅助激光解吸/电离质谱成像估计了饮食中海藻油二十二碳六烯酸 (DHA) 的迁移。向雄性斑马鱼施用 1-C-2,2-D 标记的 DHA((+3)DHA)乙酯，以评估其在体内的积累、迁移和分布。在给予(+3)DHA 10 和 15 d 后，斑马鱼体内的(+3)DHA 含量明显高于对照组。在给予(+3)DHA 5 和 10 d 的斑马鱼肠道中观察到(+3)DHA 是磷脂酰胆碱 (PC) 的组成部分。在给予(+3)DHA 1 d 的斑马鱼中，(+3)DHA 含量的 PC 倾向于在肠道中积累，表明 (+3)DHA 从乙酯到 PC 的重组在肠道中迅速发生。给予 15 d 后，(+3)DHA 含量的 PC 在全身斑马鱼的肠道、肝脏和肌肉中积累。相比之下，在大脑中未检测到含有(+3)DHA 的 PC。这些结果表明，膳食 DHA 最初作为肠细胞膜的结构成分构建到 PC 中，并逐渐迁移到肌肉等外周组织中。
