[FEATURES OF THE RELATIONSHIP BETWEEN IMMUNOLOGICAL PARAMETERS, LEVEL OF VIRAL LOAD AND SEVERITY OF MORPHOLOGICAL CHANGES IN THE LIVER ACCORDING TO NON-INVASIVE TESTS IN HBEAG--NEGATIVE PATIENTS WITH CHRONIC HEPATITIS B].

Aim - to investigate the relationship between immunological parameters, level of viral load and severity of morphological changes in the liver according to non-invasive tests in HBeAg-negative patients with chronic hepatitis B. The study included 55 HBeAg-negative patients with chronic hepatitis B. Age of patients was from 20 to 78 years. There were 20 women and 35 men. Quantitative content of HBV-DNA, HBeAg and anti-HBe, TNF-α and IFN-γ was determined in all patients› blood. Statistical processing was performed in Statistica 13 for Windows (StatSoft Inc., No. JPZ804I382130ARCN10-J). The study found an association between HBeAg/anti-HBe seroconversion and severity of liver fibrosis. It is shown that in the presence of liver fibrosis F 2-4 degree the frequency of seroconversion is lower compared with HBeAg-negative patients with F 0-1 (85,7 % vs. 100 %, p <0,05). The association of viral load above HBV-DNA >20000 IU/ml with a high degree of necro-inflammatory activity has also been shown. In HBeAg-negative patients in the presence of activity A 2-3 viral load >20,000 IU/ml is detected in 57,1 % against 20,8 % of patients with A 0-1 (p <0,05). However, it was shown that in HBeAg-negative patients with chronic HBV, TNF-α level in serum, regardless of the severity of morphological changes in the liver is higher, and IFN-γ level is not statistically different from that of healthy people. In HBeAg-negative patients with CHB the detection rate of HBeAg / anti-HBe seroconversion is significantly higher in fibrosis F 0-1. The viral load above the HBV-DNA level>20,000 IU / ml has a relationship with necroinflammatory activity, significantly higher with A 2-3. The TNF-α content is higher than in healthy people (p <0.05), and has no significant relationship with the severity of morphological changes in the liver according to non-invasive tests. The content of IFN-γ in the blood serum of HBeAg-negative patients with CHB does not statistically differ from those of healthy people.