益生菌联合应用行为分析治疗自闭症谱系障碍儿童的效果：一项前瞻性随机对照试验。
Effect of probiotics combined with applied behavior analysis in the treatment of children with autism spectrum disorder: a prospective randomized controlled trial.
机构信息
Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, China (Email: yqli1314@163. com).
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2021 Nov 15;23(11):1103-1110. doi: 10.7499/j.issn.1008-8830.2108085.
OBJECTIVES
To study the effect of probiotics combined with applied behavior analysis (ABA) in the treatment of children with autism spectrum disorder (ASD).
METHODS
A total of 41 children with ASD who attended the Affiliated Hospital of Jiangsu University from May 2019 to December 2020 were enrolled and randomly divided into an observation group with 21 children and a control group with 20 children. The children in the observation group were given oral probiotics combined with ABA intervention, while those in the control group were given ABA intervention alone. The treatment outcomes were compared between the two groups. Autism Treatment Evaluation Checklist (ATEC) was used to evaluate the severity of behavioral symptoms in both groups before intervention and at 3 months after intervention. The fecal samples were collected to analyze the difference in intestinal flora between the two groups based on 16s rRNA high-throughput sequencing.
RESULTS
Before intervention, there was no significant difference in the ATEC score between the observation and control groups (>0.05). At 3 months after intervention, both groups had a significant reduction in the ATEC score, and the observation group had a significantly lower ATEC score than the control group (<0.05). Before intervention, there was no significant difference in the composition of intestinal flora between the observation and control groups. At 3 months after intervention, there was a significant difference in the composition of intestinal flora between the observation and control groups. Compared with the control group, the observation group had significantly higher relative abundances of , , , , , and (<0.05) and significantly lower relative abundances of and (<0.05).
CONCLUSIONS
Probiotics may improve the effect of conventional ABA intervention in children with ASD by regulating intestinal flora.
目的
研究益生菌联合应用行为分析（ABA）治疗儿童孤独症谱系障碍（ASD）的效果。
方法
选取 2019 年 5 月至 2020 年 12 月在江苏大学附属医院就诊的 41 例 ASD 患儿为研究对象，采用随机数字表法分为观察组（n=21）和对照组（n=20）。观察组患儿给予口服益生菌联合 ABA 干预，对照组患儿仅给予 ABA 干预。比较两组患儿的治疗效果。于干预前及干预 3 个月后，采用孤独症治疗评估量表（ATEC）评估两组患儿行为症状严重程度。采集粪便标本，采用 16s rRNA 高通量测序分析两组患儿肠道菌群的差异。
结果
干预前，观察组与对照组的 ATEC 评分比较，差异无统计学意义（P>0.05）。干预 3 个月后，两组 ATEC 评分均较干预前降低，且观察组 ATEC 评分低于对照组，差异均有统计学意义（P<0.05）。干预前，观察组与对照组肠道菌群组成比较，差异无统计学意义（P>0.05）。干预 3 个月后，观察组与对照组肠道菌群组成比较，差异有统计学意义。与对照组比较，观察组 、 、 、 、 相对丰度升高， 、 相对丰度降低，差异均有统计学意义（P<0.05）。
结论
益生菌可能通过调节肠道菌群改善 ASD 患儿常规 ABA 干预的效果。
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