Chan S M, Hoffer P B, Maric N, Duray P
J Nucl Med. 1987 Aug;28(8):1303-7.
The effect of an anti-human transferrin receptor (anti-TFR) monoclonal antibody (MoAb), designated B3/25, and an anti-melanoma antibody, designated 96.5, on the uptake of gallium-67 (67Ga) by tumor was studied. Three groups of six athymic mice bearing a human melanoma were injected via tail vein with (a) 0.55 mg human serum albumin (HSA) (control group), (b) 0.5 mg MoAb B3/25 + 0.55 mg HSA, and (c) 0.5 mg MoAb 96.5 + 0.55 mg HSA, respectively. Twenty-four hours later, each mouse was given an intravenous dose of 5 microCi [67Ga] citrate. Biodistribution of activity (percent injected dose per gram) determined 48 hr after injection of 67Ga showed a 75% decrease in tumor uptake in the group of mice that received B3/25 (anti-TFR MoAb) compared with the control group. In contrast, MoAb 96.5 did not show any effect on melanoma uptake of 67Ga. Histologic findings suggest that the decreased uptake was not due to cellular damage resulting from binding of B3/25 to TFR. The results of this study strongly suggest the involvement of TFR in the in vivo tumor uptake of 67Ga.
研究了一种名为B3/25的抗人转铁蛋白受体（anti-TFR）单克隆抗体（MoAb）和一种名为96.5的抗黑色素瘤抗体对肿瘤摄取镓-67（67Ga）的影响。三组各有六只荷人黑色素瘤的无胸腺小鼠经尾静脉注射：（a）0.55毫克人血清白蛋白（HSA）（对照组），（b）0.5毫克MoAb B3/25 + 0.55毫克HSA，以及（c）0.5毫克MoAb 96.5 + 0.55毫克HSA。24小时后，每只小鼠静脉注射5微居里的[67Ga]柠檬酸盐。注射67Ga后48小时测定的放射性生物分布（每克注射剂量的百分比）显示，与对照组相比，接受B3/25（抗-TFR MoAb）的小鼠组肿瘤摄取量降低了75%。相比之下，MoAb 96.5对黑色素瘤摄取67Ga没有任何影响。组织学结果表明，摄取量降低并非由于B3/25与TFR结合导致的细胞损伤。本研究结果强烈提示TFR参与了67Ga在体内的肿瘤摄取。
