Wright J W, Sullivan M J, Quirk W S, Batt C M, Harding J W
J Hypertens Suppl. 1986 Dec;4(6):S408-11.
The effect of acute intracerebroventricular (i.c.v.) injections of angiotensin II and III (ANG II and ANG III; 0, 1, 10 and 100 pmol in 2 microliters artificial cerebrospinal fluid (CSF) on blood pressure and water consumption was investigated in Okamoto-Aoki spontaneously hypertensive rats (SHR), and Wistar-Kyoto (WKY) and Sprague-Dawley (SD) normotensive controls. Heightened sensitivity to i.c.v. ANG II and ANG III was observed in the SHR compared with the WKY and SD strains (P less than 0.001), for both pressor and drinking responses. In addition, i.c.v. treatment with an aminopeptidase B inhibitor, bestatin (20 nmol in 1 microliter artificial CSF) significantly potentiated the heightened pressor response to i.c.v.-injected ANG II and ANG III (100 pmol) in SHR and to a lesser degree in WKY animals compared with SD controls (P less than 0.001). These results suggest that a dysfunction in central aminopeptidase activity results in an extended life of endogenous angiotensins, and perhaps other peptides that may contribute to the high blood pressure seen in this animal model of human essential hypertension.
在冈本-青木自发性高血压大鼠（SHR）以及Wistar-Kyoto（WKY）和Sprague-Dawley（SD）正常血压对照大鼠中，研究了经脑室内（i.c.v.）注射血管紧张素II和III（ANG II和ANG III；2微升人工脑脊液（CSF）中分别含0、1、10和100皮摩尔）对血压和水消耗的影响。与WKY和SD品系相比，在SHR中观察到对i.c.v. ANG II和ANG III的敏感性增强（P < 0.001），无论是升压反应还是饮水反应。此外，与SD对照相比，用氨肽酶B抑制剂贝司他汀（1微升人工CSF中含20纳摩尔）进行i.c.v.处理可显著增强SHR对i.c.v.注射的ANG II和ANG III（100皮摩尔）的升压反应增强，在WKY动物中增强程度较小（P < 0.001）。这些结果表明，中枢氨肽酶活性的功能障碍导致内源性血管紧张素以及可能其他有助于人类原发性高血压该动物模型中高血压的肽类寿命延长。
