Pohl M, Carayon A, Laguzzi R, Nosjean A, Simonnet G, Hamon M, Legrand J C, Cesselin F
J Hypertens Suppl. 1986 Dec;4(6):S446-9.
Angiotensin II (ANG II)-like material was detected in acid extracts of rat brain using radio-immunoassay (RIA) and a radioreceptor assay (RRA). This material, expressed as ANG II equivalents, corresponded to 131 +/- 20 fmol/g and 33 +/- 4 pmol/g as assessed by RIA and RRA respectively. Such quantitative differences indicated that the brain material did not behave as authentic ANG II in both assays, and further chromatographic investigations confirmed this inference. In particular, gel filtration through Sephadex G-25 and TSK Spherogel 3000 SW revealed that the apparent molecular weight of ANG II-like material was much higher (approximately 5000-7000) than that of authentic ANG II. Furthermore, in contrast to the marked hypertension due to ANG II, a decrease in blood pressure (BP) was observed in rats following the systemic administration of chromatographic eluates enriched with brain ANG II-like material.
采用放射免疫分析法（RIA）和放射受体分析法（RRA）在大鼠脑的酸提取物中检测到了血管紧张素II（ANG II）样物质。以ANG II当量表示，通过RIA和RRA评估，该物质分别相当于131±20 fmol/g和33±4 pmol/g。这种定量差异表明，在这两种分析方法中，脑内物质的行为与真实的ANG II不同，进一步的色谱研究证实了这一推断。特别是，通过Sephadex G - 25和TSK Spherogel 3000 SW进行凝胶过滤显示，ANG II样物质的表观分子量比真实的ANG II高得多（约5000 - 7000）。此外，与ANG II引起的明显高血压相反，给大鼠全身注射富含脑ANG II样物质的色谱洗脱液后，观察到血压（BP）下降。
