HIV相关疾病中特异性免疫复合物的特征分析。

Characterization of specific immune complexes in HIV-related disorders.

作者信息

Carini C, Mezzaroma I, Scano G, D'Amelio R, Matricardi P, Aiuti F

出版信息

Scand J Immunol. 1987 Jul;26(1):21-8. doi: 10.1111/j.1365-3083.1987.tb02230.x.

DOI:10.1111/j.1365-3083.1987.tb02230.x

Abstract

Eighty-seven seropositive subjects with HIV (human immunodeficiency virus) infection together with 20 normal controls with no history of any illness were investigated for the presence of circulating immune complexes (CIC) by the conglutinin binding assay (KgBA) and further studied for isotype characterization of CIC. Six out of 87 patients showing very high values for immune complexes (CIC) were studied for the presence of free antigen. In 3 out of 6 (1, IVc1; 1, III; 1, IVa) we could detect by ultracentrifugation analysis the presence of specific HIV (p15) anti-HIV (anti-p15) and gp41-anti-gp41 CIC. Evidence in favour of this finding is supported by: the presence of specific CIC (p15-anti-p15 or gp41-anti-gp41) seen only at pH 7.2; the apparent presence of free antigen and specific HIV antibodies were only at pH 4.0. The relevance of this finding lies in the attempt to explain the occurrence of false seronegativity seen occasionally in symptomatic patients. Thus, the presence of CIC might perhaps interfere in the routine assay (i.e. ELISA) making the diagnosis difficult. All these considerations will have to be taken into account in the future handling of this disease.

摘要

对87名HIV（人类免疫缺陷病毒）感染血清反应阳性的受试者以及20名无任何疾病史的正常对照者进行了研究，采用胶固素结合试验（KgBA）检测循环免疫复合物（CIC）的存在，并进一步研究CIC的同种型特征。对87名免疫复合物（CIC）值非常高的患者中的6名进行了游离抗原存在情况的研究。在6名患者中的3名（1名，IVc1；1名，III；1名，IVa）中，通过超速离心分析我们检测到了特异性HIV（p15）抗HIV（抗p15）和gp41 - 抗gp41 CIC的存在。支持这一发现的证据有：仅在pH 7.2时可见特异性CIC（p15 - 抗p15或gp41 - 抗gp41）的存在；游离抗原和特异性HIV抗体仅在pH 4.0时明显存在。这一发现的相关性在于试图解释有症状患者偶尔出现的假血清阴性的情况。因此，CIC的存在可能会干扰常规检测（即ELISA），使诊断变得困难。在未来处理这种疾病时，所有这些因素都必须予以考虑。