Clin Lab. 2021 Nov 1;67(11). doi: 10.7754/Clin.Lab.2021.210233.
CircPVT1's effects and mechanisms of acute lymphoblastic leukemia were explored in this research.
Real-time fluorescent quantitative PCR was utilized to test circPVT1 and miR-125b in ALL samples and ALL cell systems. Dual luciferase reporter assay verified the combination of circPVT1 and miR-125b. We utilized circPVAT1 as well as miR-125b's over-expression and low-expression to prove their influence on cell proliferation and invading.
We found that more expression of circPVT1 occurred in ALL samples and ALL cell systems. CircPVT1 over-expression promoted ALL cell proliferation and migration besides invading. CircPVT1 low expression inhibited ALL cell proliferation and migration besides invading. MiR-125b was a target combination of circPVT1. CircPVT1 was able to enhance NF-κB signal pathway's expression through reducing miR-125b.
CircPVT2 can promote ALL cell proliferation and invading through miR-125b modulation of NF-κB, which would be one new potential target for ALL therapy.
本研究旨在探讨 circPVT1 对急性淋巴细胞白血病的作用及其机制。
采用实时荧光定量 PCR 检测 ALL 样本和 ALL 细胞系中的 circPVT1 和 miR-125b。双荧光素酶报告基因实验验证 circPVT1 与 miR-125b 的结合。利用 circPVT1 过表达和低表达以及 miR-125b 的过表达和低表达来证明它们对细胞增殖和侵袭的影响。
我们发现 ALL 样本和 ALL 细胞系中 circPVT1 的表达水平更高。circPVT1 过表达除了促进细胞侵袭外，还促进了 ALL 细胞的增殖和迁移。circPVT1 低表达除了抑制细胞侵袭外，还抑制了 ALL 细胞的增殖和迁移。miR-125b 是 circPVT1 的一个靶组合。circPVT1 通过降低 miR-125b 来增强 NF-κB 信号通路的表达。
circPVT2 可以通过调节 NF-κB 促进 ALL 细胞的增殖和侵袭，这可能成为 ALL 治疗的一个新的潜在靶点。
