缺氧诱导因子 1α 诱导分化的胚胎软骨细胞 1 促进口腔鳞状细胞癌细胞系的细胞迁移。

Differentiated embryo chondrocyte 1, induced by hypoxia-inducible factor 1α, promotes cell migration in oral squamous cell carcinoma cell lines.

机构信息

Department of Oral and Maxillofacial Surgery, Centre of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China; Research Center of Oral and Maxillofacial Tumor, Xiangya Hospital, Central South University, Changsha, Hunan, China; Institute of Oral Cancer and Precancerous Lesions, Central South University, Changsha, Hunan, China.

Department of Oral and Maxillofacial Surgery, Centre of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China; Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol. 2022 Feb;133(2):199-206. doi: 10.1016/j.oooo.2021.08.022. Epub 2021 Aug 31.

DOI:10.1016/j.oooo.2021.08.022

PMID:34758939

Abstract

OBJECTIVE

This study aimed to explore the correlation between differentiated embryo chondrocyte 1 (DEC1) and hypoxia-inducible factor 1α (HIF-1α) in oral squamous cell carcinoma (OSCC) and how they participate in tumor progression.

STUDY DESIGN

An immunohistochemical staining method was used to detect the expression of HIF-1α and DEC1 in 64 OSCC specimens, and the correlation between HIF-1α and DEC1 was analyzed. The expression of HIF-1α and DEC1 in OSCC cells under normoxic and hypoxic environments was assessed and analyzed by Western blotting and immunofluorescence. Furthermore, the DEC1 gene was silenced by siRNA and treated with cobalt chloride (CoCl) to analyze the effects that DEC1 and hypoxia might have on the migration ability of OSCC cells.

RESULTS

The expression of HIF-1α and DEC1 in OSCC was positively correlated. Using CoCl to simulate a hypoxic environment increased the protein levels of HIF-1α and DEC1 in OSCC cells. The HIF-1α inhibitor LW6 decreased HIF-1α and DEC1 expression in OSCC cells in a hypoxic environment. Silencing the DEC1 gene reduced the migration ability of OSCC cells.

CONCLUSION

The hypoxic environment in OSCC could upregulate the expression of DEC1 by increasing the protein level of HIF-1α, and this process might be involved in the migration of tumor cells.

摘要

目的

本研究旨在探讨分化胚胎软骨细胞 1（DEC1）与缺氧诱导因子 1α（HIF-1α）在口腔鳞状细胞癌（OSCC）中的相关性及其在肿瘤进展中的作用。

研究设计

采用免疫组织化学染色法检测 64 例 OSCC 标本中 HIF-1α和 DEC1 的表达情况，并分析 HIF-1α和 DEC1 之间的相关性。采用 Western blot 和免疫荧光法检测缺氧环境下 OSCC 细胞中 HIF-1α和 DEC1 的表达，并分析 DEC1 基因沉默和钴氯化物（CoCl）处理对 OSCC 细胞迁移能力的影响。