Furmaniak J, Nakajima Y, Hashim F A, Creagh F M, Davies Jones E, Howells R D, McLachlan S M, Rees Smith B
Acta Endocrinol Suppl (Copenh). 1987;281:157-65. doi: 10.1530/acta.0.114s157.
Studies of the TSH receptor using affinity labelling with photoactive derivatives of TSH and analysis by SDS-PAGE have shown that the receptor contains 2 subunits (A and B), linked by a disulphide bridge. Similar results are obtained with TSH receptors from human, porcine and guinea pig thyroid tissue and from guinea pig fat. Analysis of affinity labelled receptors under non-denaturing conditions suggest that subunits additional to the A and B subunits are not present. Hydrodynamic measurements indicate that the receptor A subunit has an approximately spherical structure (Stokes' radius 70 A) and when this interacts with TSH (an elongated structure with Stokes' radius 56A) a very elongated complex (Stokes' radius 104A) is formed. Isoelectric focusing studies of the TSH receptor A subunit, TSH and TSH receptor antibodies indicate that charge-charge interactions are of considerable importance in the binding of hormone and antibody to the receptor.
使用促甲状腺激素（TSH）的光活性衍生物进行亲和标记并通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分析对TSH受体的研究表明，该受体包含两个通过二硫键连接的亚基（A和B）。从人、猪和豚鼠甲状腺组织以及豚鼠脂肪中获得的TSH受体也得到了类似结果。在非变性条件下对亲和标记受体的分析表明，除A和B亚基外不存在其他亚基。流体动力学测量表明，受体A亚基具有近似球形的结构（斯托克斯半径70埃），当它与TSH（具有斯托克斯半径56埃的细长结构）相互作用时，会形成一个非常细长的复合物（斯托克斯半径104埃）。对TSH受体A亚基、TSH和TSH受体抗体的等电聚焦研究表明，电荷 - 电荷相互作用在激素和抗体与受体的结合中具有相当重要的作用。
