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用于检测甘蓝变种上位性基因效应的三重测交分析:一种更新的方法。

A triple test cross analysis to detect epistatic gene effects in cabbage var. : An updated methodology.

作者信息

Jabeen Asma, Chadha Sanjay

机构信息

CSK Himachal Pradesh Agricultural University, Palampur 176061, India.

出版信息

Saudi J Biol Sci. 2021 Nov;28(11):6153-6157. doi: 10.1016/j.sjbs.2021.06.067. Epub 2021 Jun 26.

DOI:10.1016/j.sjbs.2021.06.067
PMID:34759739
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8568707/
Abstract

The cross-pollinated crop var. shows good heterotic heterosis at high output; better standing of the plants; early maturity; larger and more homogeneous heads; consistency of head compactness; and disease-tolerance in F1 hybrids. There is very limited information documented on the epistasis of essential cabbage characters. We expand the research in this study to include an upgraded test to cross-design for enrolling and estimating epistasis and other genetic variance components controlling head yield and component traits in cabbage. The data was obtained from 45 families produced by crossing 15 lines with three testers; SC 2008-09, E-1-3-1&2, and their single cross F1, was subjected to triple test cross analysis. The current study results confirmed "j + 1" form of epistasis which is a major component for all traits. The plant spread, non-wrapper leaves, nethead/grossweight, polar/equatorial diameter, marketable head yield per plot, iron content and dry matter lugged both "j + 1" and 'i' type with the predominance of the 'i' type of interaction. Except for head shape index, equatorial diameter, head compactness was more noticeable when observed in dominance component. The degree of dominance is in the partial range, but both the head shape index/compactness and equatorial diameter showed over dominance. For maximum part, superiority was shown in both the directions. Appropriate breeding procedures are proposed to exploit the different forms of gene effects discovered for genetic improvement of head yield and quality traits.

摘要

杂交授粉作物品种在高产时表现出良好的杂种优势;植株生长势较好;早熟;花球更大且更均匀;花球紧实度一致;F1代杂种具有抗病性。关于甘蓝重要性状上位性的文献记载非常有限。我们在本研究中扩大了研究范围,包括升级杂交设计试验,以登记和估计控制甘蓝花球产量及构成性状的上位性和其他遗传方差成分。数据来自15个品系与3个测验种杂交产生的45个家系;对SC 2008 - 09、E - 1 - 3 - 1&2及其单交F1进行了三重测交分析。当前研究结果证实了“j + 1”形式的上位性是所有性状的主要成分。植株开展度、外叶数、净菜重/毛重、极径/赤道径、每小区可上市花球产量、铁含量和干物质含量同时表现出“j + 1”和“i”型互作,且以“i”型互作为主。除花球形状指数和赤道径外,在显性成分中观察到花球紧实度更为明显。显性程度处于部分显性范围,但花球形状指数/紧实度和赤道径均表现出超显性。在大多数情况下,两个方向均表现出优势。提出了适当的育种程序,以利用所发现的不同形式的基因效应来改良花球产量和品质性状的遗传。

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