Ahmad Saeed, Khan Sanaullah, Zeb Alam, Shah Syed Wadood Ali, Ahmad Bashir, Khan Ayaz Ali, Ali Waqar, Khan Nadir Zaman, Zamani Ghazala Yasmin
Department of Zoology, University of Malakand, Chakdara, Khyber Pakhtunkhwa, Pakistan.
Department of Zoology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan.
Saudi J Biol Sci. 2021 Nov;28(11):6352-6358. doi: 10.1016/j.sjbs.2021.06.095. Epub 2021 Jul 8.
The analgesic, antidiarrheal, and neuro-pharmacological potentials of leaves extract were screened in animal models. Potential analgesic response was noted (*P < 0.05, P < 0.01, P < 0.001) in formalin, acetic acid and heat-induced pain models in a dose-dependent manner. Maximum activity by means of writhing inhibition was documented for at 300 mg/kg that was found to be 71.79% (17.43 ± 1.31). In first phase, the at a dose of 150 and 300 mg/kg showed analgesic activity and reduced the pain by 54.18% (18.39 ± 1.67) and 62.90% (14.89 ± 1.56), respectively. In second phase, the at a dose of 150 and 300 mg/kg showed analgesic activity and reduced the pain by 69.48% (19.78 ± 1.44) and 70.89% (18.86 ± 1.58), respectively. In hot plate method, the at a dose of 150 and 300 mg/kg showed the maximum response of 61.16% (8.47 ± 1.23) and 67.39% (10.09 ± 1.04), respectively at 60 min. Scopolamine significantly reduces spontaneous alteration in Y-maze model for antiamnesic activity. significantly increased the discrimination index in a dose-dependent manner using novel object recognition test (NORT) model. Exploration time in sec for the novel object was increased significantly (P < 0.001) by donepezil decreased for familiar one with a discrimination index (DI) of 62.18%. significantly increased the discrimination index by 60.86% and 57.24% at 300 and 150 mg/kg b.w, respectively. The lowest DI of 53.80% at 75 mg/kg was observed in comparison to the amnesic group. The significant decreased the elevated levels of acetylcholinesterase (AChE) and malondialdehyde (MDA and enhancing level of acetylcholine (ACh), superoxide dismutase (SOD) and catalase (CAT) acting as an antioxidant agent. reduced the total number of diarrheal feces to lesser extent at dose-dependent manner. From the study results, it is suggested that the extract possess good analgesic and antiamnesic activity however the antidiarrheal effects of plant were negligible. In the current study, the traditional use of the plant as a source of medicine has been validated.
在动物模型中对树叶提取物的镇痛、止泻和神经药理学潜力进行了筛选。在福尔马林、乙酸和热诱导疼痛模型中，观察到潜在的镇痛反应（P < 0.05，P < 0.01，P < 0.001）呈剂量依赖性。通过扭体抑制法记录到，在300 mg/kg剂量时活性最高，扭体抑制率为71.79%（17.43 ± 1.31）。在第一阶段，150和300 mg/kg剂量的提取物显示出镇痛活性，疼痛减轻率分别为54.18%（18.39 ± 1.67）和62.90%（14.89 ± 1.56）。在第二阶段，150和300 mg/kg剂量的提取物显示出镇痛活性，疼痛减轻率分别为69.48%（19.78 ± 1.44）和70.89%（18.86 ± 1.58）。在热板法中，150和300 mg/kg剂量的提取物在60分钟时最大反应率分别为61.16%（8.47 ± 1.23）和67.39%（10.09 ± 1.04）。东莨菪碱在Y迷宫模型中显著降低自发交替行为以发挥抗遗忘活性。提取物使用新物体识别测试（NORT）模型以剂量依赖性方式显著提高辨别指数。多奈哌齐使新物体的探索时间显著增加（P < 0.001），熟悉物体的探索时间减少，辨别指数（DI）为62.18%。提取物在300和150 mg/kg体重剂量时分别使辨别指数显著提高60.86%和57.24%。与遗忘组相比，75 mg/kg剂量时最低辨别指数为53.80%。提取物显著降低升高的乙酰胆碱酯酶（AChE）和丙二醛（MDA）水平，并提高乙酰胆碱（ACh）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平，起到抗氧化剂的作用。提取物以剂量依赖性方式在较小程度上减少腹泻粪便总数。从研究结果来看，表明该提取物具有良好的镇痛和抗遗忘活性，然而该植物的止泻作用可忽略不计。在当前研究中，该植物作为药物来源的传统用途得到了验证。
