一种c-ras-Ki癌基因在人骨肉瘤细胞系中被激活、扩增并过度表达。

A c-ras-Ki oncogene is activated, amplified and overexpressed in a human osteosarcoma cell line.

作者信息

Nardeux P C, Daya-Grosjean L, Landin R M, Andéol Y, Suàrez H G

出版信息

Biochem Biophys Res Commun. 1987 Jul 31;146(2):395-402. doi: 10.1016/0006-291x(87)90542-0.

Abstract

We present a characterization of an activated oncogene which we found to be present in DNA of the OHA osteosarcoma cell line. We identify this tumor oncogene which transforms Swiss mouse 3T3-cells, with c-ras-Ki 2, one of two known members of the Kirsten ras family of human proto-oncogenes. Its structural outlines are given and we show that: 1) a single point mutation causing a substitution of valine for glycine in codon 12 was found by DNA sequencing; 2) the c-ras-Ki gene is amplified and overexpressed in the original OHA tumor cells and its transformants and 3) the gene product is an abnormal form of the p21 protein.

摘要

我们展示了一种活化癌基因的特征，该基因存在于OHA骨肉瘤细胞系的DNA中。我们鉴定出这种能转化瑞士小鼠3T3细胞的肿瘤癌基因，它与c-ras-Ki 2相同，c-ras-Ki 2是人类原癌基因Kirsten ras家族两个已知成员之一。文中给出了其结构概述，并且我们发现：1）通过DNA测序发现单个点突变导致密码子12处的甘氨酸被缬氨酸取代；2）c-ras-Ki基因在原始OHA肿瘤细胞及其转化细胞中扩增并过度表达；3）该基因产物是p21蛋白的异常形式。