Dorn A, Bollekens J, Staub A, Benoist C, Mathis D
Cell. 1987 Sep 11;50(6):863-72. doi: 10.1016/0092-8674(87)90513-7.
NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y(star) that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.
NF-Y是一种序列特异性DNA结合蛋白，可识别Y盒，这是所有主要组织相容性复合体II类基因共有的启动子元件。由于14碱基的Y元件反向包含一个CCAAT盒，我们不禁要问NF-Y是否实际上是一种CCAAT盒结合蛋白，以及它是否与先前描述的CCAAT结合因子CBP和CTF/NF-I相关。凝胶阻滞、甲基化干扰、饱和诱变和交叉竞争实验的数据明确证实，NF-Y是一个完全不同的CCAAT盒结合实体。此外，这些实验还发现了一种与胸苷激酶启动子相互作用的第四个CCAAT结合蛋白NF-Y(star)。显然，存在多种识别CCAAT序列的因子；现在迫切需要了解这种多样性的功能意义。
