Bioconversion of Apple Pomace into Microbial Protein Feed Based on Extrusion Pretreatment.

Apple pomace (AP) is often used directly as animal feed, while the value of feeding is limited by its low protein content. In this study, extrusion pretreatment was performed for AP, and further fermentation was carried out to improve its nutrition value. Strains suitable for extruded apple pomace (EAP) to produce high-quality microbial protein (MP) feed were screened from 12 different strains. Results showed that Aspergillus niger 3.324 (Asn), Candida utilis1314 (Cau), Geotrichum candidum 1315 (Gec), Bacillus subtilis A308 (Bas1), and Lactic acid bacteria (Lac) were screened as the dominant strains, which exhibited higher feeding value. Strong symbiotic effect was observed in fermentation with mixed strains of Asn, Cau, Gec, and Lac at the ratio of 1:1:1:1. Compared with AP, the pure protein content in the optimized fermented EAP (FEAP) was increased by 138% accompanying with a pleasant flavor and taste. And its pure protein content was increased by 19.20% in comparison to that of the fermented apple pomace. The nutrition value of FEAP was characterized by amino acid profiles; it found that FEAP was comparable to other commercial proteins with higher contents of histidine, phenylalanine, threonine, and valine. Combination of fermentation and extrusion technology significantly enhanced pure protein content and nutritional composition of apple pomace, which was revalorized as a nutritive animal feed rich in microbial protein.