Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China; School of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Ann Palliat Med. 2021 Oct;10(10):10391-10400. doi: 10.21037/apm-21-2318.
Type 2 diabetes mellitus (T2DM) is a major social and public health problem which may be induced by intestinal flora imbalance through inflammatory response, and the specific mechanism remains unclear. In this study, we aim to explore the interaction network of intestinal flora and cell inflammation in T2DM.
This a case-control study. Patients with T2DM was the case group and healthy people as control. The differences of cytokine expression levels between patients with T2DM and healthy controls were assessed by using flow cytometry. The diversity and abundance of intestinal flora were evaluated by using 16S rRNA three-generation full-length sequencing technology.
A total of 29 patients with T2DM and 28 healthy controls were included for analysis. Compared with the healthy control group, the expression levels of plasma cytokine interleukin-2 (IL-2) (P=0.0000006), IL-6 (P=0.000193), tumor necrosis factor α (TNF-α) (P=0.016), interferon-γ (IFN-γ) (P=0.000036) and interleukin-17 (IL-17) (P=0.004) were significantly up-regulated in T2DM patients, and the abundance of Megamonas_funiformis (P=0.0016) and Escherichia (P=0.049) in the intestine were significantly increased. In contrast, the abundance of Bacteroides_stercoris (P=0.0068), Bacteroides_uniformis (P=0.033), and Phascolarctobacterium_faecium (P=0.033) were decreased in T2DM patients. Further, differentially expressed Escherichia had a positive correlation with IFN-γ (r=0.73) by Pearson correlation analysis.
The interaction network between the intestinal bacteria Escherichia and the cytokine IFN-γ may drive inflammation in visceral adipose tissue (VAT), indicating insulin signal transduction can be inhibited in adipocytes to induce insulin resistance.
2 型糖尿病（T2DM）是一个主要的社会和公共卫生问题，可能通过炎症反应引起肠道菌群失衡，但其具体机制尚不清楚。本研究旨在探索 T2DM 中肠道菌群与细胞炎症的相互作用网络。
这是一项病例对照研究。T2DM 患者为病例组，健康人群为对照组。采用流式细胞术检测 T2DM 患者与健康对照者细胞因子表达水平的差异。采用 16S rRNA 三代全长测序技术评估肠道菌群的多样性和丰度。
共纳入 29 例 T2DM 患者和 28 例健康对照者进行分析。与健康对照组相比，T2DM 患者血浆细胞因子白细胞介素-2（IL-2）（P=0.0000006）、IL-6（P=0.000193）、肿瘤坏死因子-α（TNF-α）（P=0.016）、干扰素-γ（IFN-γ）（P=0.000036）和白细胞介素-17（IL-17）（P=0.004）表达水平显著升高，肠道内 Megamonas_funiformis（P=0.0016）和 Escherichia（P=0.049）丰度显著增加。相反，T2DM 患者肠道内 Bacteroides_stercoris（P=0.0068）、Bacteroides_uniformis（P=0.033）和 Phascolarctobacterium_faecium（P=0.033）丰度降低。进一步通过 Pearson 相关分析发现，差异表达的 Escherichia 与 IFN-γ呈正相关（r=0.73）。
肠道细菌 Escherichia 与细胞因子 IFN-γ 之间的相互作用网络可能驱动内脏脂肪组织（VAT）的炎症，表明胰岛素信号转导可在脂肪细胞中被抑制，从而导致胰岛素抵抗。
