Kudo Noritaka, Kudoh Shinji, Matsuo Akira, Motooka Yamato, Ito Takaaki
Department of Pathology and Experimental Medicine, Kumamoto University Graduate School of Medical Sciences, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
Department of Pathology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Acta Histochem Cytochem. 2021 Oct 29;54(5):143-153. doi: 10.1267/ahc.21-00012. Epub 2021 Oct 2.
Zinc finger, myeloproliferative, and mental retardation-type containing 3 (ZMYM3) is a highly conserved protein among vertebrates. Although it promotes DNA repair and moderate histone acetylation, the other functions of ZMYM3 remain unclear. We herein examined the physiological functions of ZMYM3 in human lung cancer using a -knockdown small cell lung cancer (SCLC) cell line. -knockdown SCLC cells grew slowly and the Ki-67 labeling index was lower in -knockdown cells than in mock cells. The subcutaneous tumors that formed after xenotransplantation into immunodeficient mice were slightly smaller in the -knockdown group than in the mock group. Furthermore, public RNA-sequencing data analyses showed similar RNA profiles between and some cell proliferation markers. These results indicate that ZMYM3 promotes cell proliferation in human lung carcinomas, particularly SCLC.
含锌指、骨髓增殖和智力迟钝型3（ZMYM3）是脊椎动物中高度保守的蛋白质。尽管它促进DNA修复并适度进行组蛋白乙酰化，但ZMYM3的其他功能仍不清楚。我们在此使用敲低的小细胞肺癌（SCLC）细胞系研究了ZMYM3在人肺癌中的生理功能。敲低ZMYM3的SCLC细胞生长缓慢，敲低细胞中的Ki-67标记指数低于模拟细胞。异种移植到免疫缺陷小鼠后形成的皮下肿瘤，敲低组比模拟组略小。此外，公开的RNA测序数据分析显示ZMYM3与一些细胞增殖标志物之间的RNA谱相似。这些结果表明ZMYM3促进人肺癌尤其是SCLC中的细胞增殖。
