Department of Clinical Pharmacy, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China.
Department of Pharmacy, The Eight Hospital of Xian, Xian, China.
J Biochem Mol Toxicol. 2022 Feb;36(2):e22959. doi: 10.1002/jbt.22959. Epub 2021 Nov 12.
Long noncoding RNAs (LncRNAs) have been reported to play a vital role in the development of oesophageal squamous cell carcinoma (OSCC). Our previous study revealed that the significant upregulation of the LncRNA small nucleolar RNA host gene 6 (SNHG6) in OSCC promotes OSCC tumourigenesis. However, the mechanisms underlying the dynamics of SNHG6 expression in OSCC have rarely been studied. In this study, we verified the tumour-promoting effect of SNHG6 through sponging miR-101-3p, and their levels were negatively correlated in human samples of OSCC. In addition, miR-101-3p overexpression reversed the effect of SNHG6. Moreover, we confirmed that SNHG6/miR-101-3p affects OSCC by regulating the expression of the enhancer of zeste 2 (EZH2). The effect of EZH2 silencing resembled closely that of SNHG6 knockdown. EZH2 silencing inhibited the expression of protein cyclin D1 and β-catenin, but in contrast, it enhanced the expression of E-cadherin. These findings demonstrated the oncogenic role of SNHG6, which promotes OSCC progression by regulating the expression of EZH2 through its interaction with miR-101-3p. These findings may help in improving the diagnosis and treatment methods of OSCC.
长链非编码 RNA（lncRNA）在食管鳞状细胞癌（OSCC）的发展中起着至关重要的作用。我们之前的研究表明，lncRNA 小核仁 RNA 宿主基因 6（SNHG6）在 OSCC 中的显著上调促进了 OSCC 肿瘤发生。然而，SNHG6 在 OSCC 中表达动态的机制很少被研究。在这项研究中，我们通过海绵 miR-101-3p 验证了 SNHG6 的促瘤作用，并且它们在人 OSCC 样本中的水平呈负相关。此外，miR-101-3p 的过表达逆转了 SNHG6 的作用。此外，我们证实 SNHG6/miR-101-3p 通过调节胚胎外胚层发育蛋白 2（EZH2）的表达来影响 OSCC。EZH2 沉默的作用与 SNHG6 敲低非常相似。EZH2 沉默抑制蛋白 cyclin D1 和 β-catenin 的表达，但相反，它增强了 E-钙黏蛋白的表达。这些发现表明 SNHG6 具有致癌作用，它通过与 miR-101-3p 相互作用调节 EZH2 的表达来促进 OSCC 的进展。这些发现可能有助于改善 OSCC 的诊断和治疗方法。
