密蒙花可改善 CCl 诱导的大鼠肝内质网应激。
Indigofera linifolia ameliorated CCl induced endoplasmic reticulum stress in liver of rat.
机构信息
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Institute of Biochemistry, Biotechnology and Bioinformatics, Faculty of Sciences, The Islamia University of Bahawalpur, Pakistan.
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
出版信息
J Ethnopharmacol. 2022 Mar 1;285:114826. doi: 10.1016/j.jep.2021.114826. Epub 2021 Nov 9.
ETHNOPHARMACOLOGICAL RELEVANCE
Indigofera linifolia (L.f.) Retz. is used in subcontinent for liver disorders, in wounds, febrile eruption and as diuretic.
AIM OF STUDY
The current study evaluates the protective effects of the methanol extract of Indigofera linifolia (ILM) on CCl-induced endoplasmic reticulum (ER) stress in liver of rat.
METHODS
ILM was analyzed for phytochemical classes, total phenolic (TPC) and flavonoid content (TFC) as well as multidimensional in vitro antioxidant assays. Male (Sprague Dawley) rats were dispersed into seven groups (6 rats/group) receiving 0.9% saline (1 ml/kg bw), CCl (1 ml/kg bw) diluted in olive oil (3:7 v/v), silymarin (200 mg/kg bw) + CCl (30% v/v), ILM (150 mg/kg bw) + CCl (30% v/v), ILM (300 mg/kg bw) + CCl and ILM alone (either 150 mg/kg bw or 300 mg/kg bw).
RESULTS
ILM extract was constituted of different phytochemical classes. Co-administration of ILM along with CCl to rat revert the level of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and total bilirubin in blood serum and antioxidant parameters in liver. Further, CCl increased the level of ER stress markers and inflammatory mediators while decreased level of GCLC and Nrf-2 in liver tissues of rat. CCl-induced histopathological variations were reduced with ILM co-administration in liver tissues.
CONCLUSION
The results suggest that active phyto-constituents of I. linifolia might be responsible for its antioxidant, anti-inflammatory and gene-regulating activities.
民族药理学相关性
木蓝（L.f.）Retz. 在次大陆用于肝脏疾病、伤口、发热疹和利尿剂。
研究目的
本研究评估甲醇提取物木蓝（ILM）对 CCl 诱导的大鼠肝脏内质网（ER）应激的保护作用。
方法
分析 ILM 的植物化学类、总酚（TPC）和类黄酮含量（TFC）以及多维体外抗氧化测定。雄性（斯普拉格-道利）大鼠分为 7 组（每组 6 只），分别给予 0.9%生理盐水（1ml/kg bw）、橄榄油（3:7 v/v）稀释的 CCl（1ml/kg bw）、水飞蓟素（200mg/kg bw）+CCl（30% v/v）、ILM（150mg/kg bw）+CCl（30% v/v）、ILM（300mg/kg bw）+CCl 和单独的 ILM（150mg/kg bw 或 300mg/kg bw）。
结果
ILM 提取物由不同的植物化学类组成。ILM 与 CCl 一起给予大鼠可使血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）和总胆红素水平以及肝脏抗氧化参数恢复正常。此外，CCl 增加了 ER 应激标志物和炎症介质的水平，同时降低了肝脏组织中 GCLC 和 Nrf-2 的水平。ILM 联合给药可减少 CCl 诱导的肝脏组织的组织病理学变化。
结论
结果表明，木蓝的活性植物成分可能与其抗氧化、抗炎和基因调节活性有关。
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