Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China.
Int J Mol Sci. 2021 Oct 24;22(21):11458. doi: 10.3390/ijms222111458.
As a kind of Amaryllidaceae alkaloid which is accumulated in the species of Lycoris plants, lycorine has a range of physiological effects. The biosynthesis pathway of lycorine has been partly revealed, but the transport and accumulation mechanisms of lycorine have rarely been studied. In this study, an ATP-binding cassette (ABC) transporter from (L'Hér) Herb., namely LaABCB11, was cloned and functionally characterized. Heterologous expression showed that LaABCB11 transported lycorine in an outward direction, increased the tolerance of yeast cells to lycorine, and caused a lower lycorine accumulation in transformants than control or mutant in yeast. LaABCB11 is associated with the plasma membrane, and in situ hybridization indicated that was mainly expressed in the phloem of leaves and bulbs, as well as in the cortical cells of roots. These findings suggest that LaABCB11 functions as a lycorine transport and it might be related to the translocation and accumulation of lycorine from the leaves and bulbs to the roots.
作为石蒜科生物碱的一种，在石蒜属植物中积累，石蒜碱具有广泛的生理作用。石蒜碱的生物合成途径已经部分揭示，但石蒜碱的运输和积累机制很少被研究。在这项研究中，从 （L'Hér） Herb. 克隆并功能表征了一种 ABC 转运蛋白（ABC）转运蛋白，即 LaABCB11。异源表达表明，LaABCB11 向外运输石蒜碱，增加了酵母细胞对石蒜碱的耐受性，并导致转化体中的石蒜碱积累低于对照或突变体。LaABCB11 与质膜相关，原位杂交表明 主要在叶片和鳞茎的韧皮部以及根的皮层细胞中表达。这些发现表明，LaABCB11 作为石蒜碱的转运蛋白发挥作用，可能与石蒜碱从叶片和鳞茎向根的转运和积累有关。
