Plant Epigenetics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-son, Okinawa 904-0412, Japan.
Int J Mol Sci. 2021 Nov 4;22(21):11964. doi: 10.3390/ijms222111964.
Mangroves are salt-tolerant plant species that grow in coastal saline water and are adapted to harsh environmental conditions. In this study, we de novo assembled and functionally annotated the transcriptome of , the widely distributed mangrove from the largest mangrove family (Rhizophoraceae). The final transcriptome consists of 200,491 unigenes with an average length, and N50 of 912.7 and 1334 base pair, respectively. We then compared the genome-wide expression profiles between the two morphologically distinct natural populations of this species growing under different levels of salinity depending on their distance from the ocean. Among the 200,491 unigenes, 40,253 were identified as differentially expressed between the two populations, while 15,741 and 24,512 were up- and down-regulated, respectively. Functional annotation assigned thousands of upregulated genes in saline environment to the categories related to abiotic stresses such as response to salt-, osmotic-, and oxidative-stress. Validation of those genes may contribute to a better understanding of adaptation in mangroves species. This study reported, for the first time, the transcriptome of , and the dynamic of it in response to salt stress and provided a valuable resource for elucidation of the molecular mechanism underlying the salt stress response in mangroves and other plants that live under stress.
红树林是一种耐盐植物，生长在沿海咸水环境中，适应恶劣的环境条件。在这项研究中，我们从头组装并对 的转录组进行了功能注释， 是分布广泛的红树林物种，属于最大的红树科（Rhizophoraceae）。最终的转录组由 200491 条 unigene 组成，平均长度为 ，N50 分别为 912.7 和 1334 碱基对。然后，我们比较了两种形态截然不同的自然种群在基因组水平上的表达谱，这两种种群生长在不同盐度水平下，取决于它们与海洋的距离。在 200491 条 unigene 中，有 40253 条被鉴定为两种种群之间差异表达，其中 15741 条和 24512 条分别上调和下调。功能注释将数千个在盐环境中上调的基因分配到与非生物胁迫相关的类别中，如对盐、渗透和氧化胁迫的反应。对这些基因的验证可能有助于更好地理解红树林物种的适应。本研究首次报道了 的转录组及其对盐胁迫的反应动态，为阐明红树林和其他在胁迫下生存的植物对盐胁迫反应的分子机制提供了有价值的资源。
