School of Software, Hallym University, Chuncheon-si 24252, Korea.
Bio-IT Research Center, Hallym University, Chuncheon-si 24252, Korea.
Sensors (Basel). 2021 Oct 20;21(21):6945. doi: 10.3390/s21216945.
The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45° angle near the PCR chip, eliminating the need of additional filter wheels. The results show precise positioning of the filter wheel with an error less than 20 μm. Fluorescence detection results using a reference dye and standard DNA amplification showed comparable performance to that of the photodiode system.
多路实时 PCR 系统的便携性差和成本高限制了该设备在即时检验（POC）中的应用。为了解决这个问题，本文提出了一种紧凑且经济高效的荧光检测系统，可以集成到多路实时 PCR 设备中。该系统使用带嵌入式镜头的开放式平台相机代替光电二极管或工业相机。集成了一个使用滑动带的紧凑型滤光轮，激发 LED 以 45°角固定在 PCR 芯片附近，无需额外的滤光轮。结果表明，滤光轮的定位精度误差小于 20μm。使用参考染料和标准 DNA 扩增的荧光检测结果与光电二极管系统的性能相当。
