Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo Náměstí 542/2, 166 00 Prague 6, Czech Republic.
Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 2030/8, 128 43 Prague 2, Czech Republic.
Molecules. 2021 Oct 26;26(21):6468. doi: 10.3390/molecules26216468.
Double and triple bonds have significant effects on the biological activities of lipids. Determining multiple bond positions in their molecules by mass spectrometry usually requires chemical derivatization. This work presents an HPLC/MS method for pinpointing the double and triple bonds in fatty acids. Fatty acid methyl esters were separated by reversed-phase HPLC with an acetonitrile mobile phase. In the APCI source, acetonitrile formed reactive species, which added to double and triple bonds to form [M + CHN] ions. Their collisional activation in an ion trap provided fragments helpful in localizing the multiple bond positions. This approach was applied to fatty acids with isolated, cumulated, and conjugated double bonds and triple bonds. The fatty acids were isolated from the fat body of early-nesting bumblebee and seeds or seed oils of , , and . Using the method, the presence of the known fatty acids was confirmed, and new ones were discovered.
双键和叁键对脂质的生物活性有显著影响。用质谱法确定其分子中的多重键位置通常需要化学衍生化。本工作提出了一种高效液相色谱/质谱法，用于精确定位脂肪酸中的双键和叁键。脂肪酸甲酯通过反相高效液相色谱用乙腈流动相分离。在 APCI 源中，乙腈形成活性物质，与双键和叁键加成形成[M + CHN]离子。它们在离子阱中进行碰撞激活，提供有助于定位多重键位置的片段。该方法应用于具有孤立、累积和共轭双键和叁键的脂肪酸。脂肪酸从早期筑巢大黄蜂的脂肪体和 、 、 种子或种子油中分离出来。使用该方法证实了已知脂肪酸的存在，并发现了新的脂肪酸。
